his.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Use of N,N-diacetylchitobiose in decreasing toxic effects of indoor air pollution by preventing oxidative DNA damage
Microbial Diversity Research Centre, Dr D Y Patil Biotechnology and Bioinformatics Institute, Dr D Y Patil Vidyapeeth, Pune, India.
Microbial Diversity Research Centre, Dr D Y Patil Biotechnology and Bioinformatics Institute, Dr D Y Patil Vidyapeeth, Pune, India.
Microbial Diversity Research Centre, Dr D Y Patil Biotechnology and Bioinformatics Institute, Dr D Y Patil Vidyapeeth, Pune, India.
Department of Microbiology, Savitribai Phule University of Pune, Pune, India.
Visa övriga samt affilieringar
2016 (Engelska)Ingår i: Biologia (Bratislava), ISSN 0006-3088, E-ISSN 1336-9563, Vol. 71, nr 5, s. 505-515Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Indoor air pollution occurs due to hazardous pollutants, such as tobacco smoke, pesticides and carbon oxides, sulphur oxides and nitrogen oxides arising from combustion of biomass fuels. Exposure to these pollutants results in respiratory conditions like asthma, chronic obstructive pulmonary disease, lung cancer, pneumonia and other lower respiratory infections. Several of these infections are a result of inflammation and oxidative stress. Here we demonstrate the ability of N,N-diacetylchitobiose in preventing oxidative DNA damage in peripheral blood mononuclear cells exposed to biomass smoke extracts and cigarette smoke extract. The cytotoxic effect of these pollutants was determined by trypan blue exclusion assay in peripheral blood mononuclear cells, where cytotoxicity in decreasing order was  garette > wood > sawdust > cowdung. Cytotoxicity could be due to single- and double-strand breaks in the DNA as a result of oxidative stress. Comet assay measures the extent of DNA damage in the cells exposed to toxic agents. When mononuclear cells were treated with N,N-diacetylchitobiose and later exposed to smoke extracts, the extent of DNA damage decreased by 44.5% and 57.5% as compared to untreated cells. The protection offered by N,N-diacetylchitobiose towards oxidative DNA damage was at par with quercetin, a popular herbal medicine. Glutathione-S-transferase activity was determined in mononuclear cells exposed to smoke extracts, where oxidative stress in cells exposed to cigarette smoke extract was maximum. The present study demonstrates for the first time the ability of N,N -diacetylchitobiose to alleviate the harmful effects of indoor air pollutants.

Ort, förlag, år, upplaga, sidor
Bratislava: Springer, 2016. Vol. 71, nr 5, s. 505-515
Nyckelord [en]
Comet assay, indoor air pollution, biomass smoke, cigarette smoke, cytotoxicity
Nationell ämneskategori
Miljövetenskap Immunologi Mikrobiologi
Forskningsämne
Naturvetenskap; Bioteknik; Fysisk aktivitet, idrott, hälsa och digital teknik
Identifikatorer
URN: urn:nbn:se:his:diva-12311DOI: 10.1515/biolog-2016-0075ISI: 000379818000006Scopus ID: 2-s2.0-84976585373OAI: oai:DiVA.org:his-12311DiVA, id: diva2:932375
Projekt
COPD-project funded by SIDA
Forskningsfinansiär
Sida - Styrelsen för internationellt utvecklingssamarbeteTillgänglig från: 2016-06-01 Skapad: 2016-06-01 Senast uppdaterad: 2019-11-25Bibliografiskt granskad

Open Access i DiVA

Biologia-2-2016.pdf(540 kB)86 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 540 kBChecksumma SHA-512
1512f7292dc5a5c4fc8a6b6ab9df99dfb0928270ead25123f2857659585d45c62ea40145859565b42b796cc1126db39aadc9d936bf5926f3e734b01e55cfb260
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextScopus

Personposter BETA

Mandal, AbulEriksson, Cecilia

Sök vidare i DiVA

Av författaren/redaktören
Mandal, AbulEriksson, Cecilia
Av organisationen
Institutionen för biovetenskapForskningscentrum för SystembiologiInstitutionen för hälsa och lärandeForskningsspecialiseringen Hälsa och Lärande
I samma tidskrift
Biologia (Bratislava)
MiljövetenskapImmunologiMikrobiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 86 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 1027 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf