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A Biotechnological Approach for the Production of Red Gerbera (Gerbera Jamesonii Bolus)
Plant Breeding & Gene Engineering Lab., Dept. of Botany, Rajshahi University, Bangladesh.
Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi. (Fysiologi, farmakologi och toxikologi (P2T))ORCID-id: 0000-0002-6319-4055
Plant Breeding & Gene Engineering Lab., Dept. of Botany, Rajshahi University, Bangladesh / Dept. of Chemistry, Biochemistry & Physics, University of Quebec at Trois - Rivieres, Quebec, Canada.
Plant Breeding & Gene Engineering Lab., Dept. of Botany, Rajshahi University, Bangladesh.
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2014 (Engelska)Ingår i: Nova Journal of Medical and Biological Sciences, ISSN 2292-793X, Vol. 2, nr 1, s. 1-6Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

An in vitro propagation of a red Gerbera (Gerbera jamesonii) variety was achieved by culturing flower bud, leaf segments and flower stalk segments of 80 days old field grown plants on Murashige and Skoog (MS) medium supplemented with different concentration (1.0-6.0 mg/l) of 6-benzyl adenine (BA) in combination with single concentration (1.0 mg/l) of α-naphthalene acetic acid (NAA). Lower concentration of BA (1.0 and 2.0 mg/l) with NAA induced the explants to form callus. On the other hand when the explants were cultured in higher concentration (5.0 mg/l) of BA produced shoots and 5.0 mg/l BA with 1.0 mg/l NAA was found to be the best for shoot proliferation of the three explants optimum response was obtained from flower buds. Further multiplication of shoots occurred upon transfer of shoot clumps to BA containing MS medium. Regenerated shoots were rooted in MS medium with indole-3-acetic acid (IAA) or indole-3-buteric acid (IBA) and maximum frequency (81%) of rooting with highest number (4) of roots per shoot was achieved in MS medium fortified with 0.3 mg/l IBA. The rooted shoots were acclimatized and successfully established in soil under natural environment with maximum 84% survivability.

Ort, förlag, år, upplaga, sidor
Canada: Nova Explore Publications , 2014. Vol. 2, nr 1, s. 1-6
Nyckelord [en]
Gerbera, herbaceous flower, micro propagation, red variety, regeneration
Nationell ämneskategori
Naturvetenskap
Forskningsämne
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:his:diva-9019OAI: oai:DiVA.org:his-9019DiVA, id: diva2:714002
Tillgänglig från: 2014-04-24 Skapad: 2014-04-24 Senast uppdaterad: 2017-11-27Bibliografiskt granskad

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Mandal, Abul

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