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Proteomic analysis shows decreased Type I fibers and ectopic fat accumulation in skeletal muscle from women with PCOS
Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.ORCID iD: 0000-0002-3424-1502
Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.
Department of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Sweden.
School of Biomedical Sciences, University of New South Wales, Sydney, Australia.
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2024 (English)In: eLife, ISSN 2050-084X, Vol. 12Article in journal (Refereed) Published
Abstract [en]

Background: Polycystic ovary syndrome’s (PCOS) main feature is hyperandrogenism, which is linked to a higher risk of metabolic disorders in women. Gene expression analyses in adipose tissue and skeletal muscle reveal dysregulated metabolic pathways in women with PCOS, but these differences do not necessarily lead tochanges in protein levels and biological function. Methods: To advance our understanding of the molecular alterations in PCOS, we performed global proteomic and phosphorylation site analysis using tandem mass spectrometry. Adipose tissue and skeletal muscle were collected at baseline from 10 women with and without PCOS, and in women with PCOS after 5 weeks of treatment with electrical stimulation. Results: Perilipin-1, a protein that typically coats the surface of lipid droplets in adipocytes, was increased whereas proteins involved in muscle contraction and type I muscle fiber function were downregulated in PCOS muscle. Proteins in the thick and thin filaments had many altered phosphorylation sites, indicating differences in protein activity and function. The upregulated proteins in muscle post treatment were enriched in pathways involved in extracellular matrix organization and wound healing, which may reflect a protective adaptation to repeated contractions and tissue damage due to needling. A similar, albeit less pronounced, upregulation in extracellular matrix organization pathways was also seen in adipose tissue. Conclusions: Our results suggest that hyperandrogenic women with PCOS have higher levels of extramyocellular lipids and fewer oxidative insulin-sensitive type I muscle fibers. These could be key factors leading insulin resistance in PCOS muscle while electric stimulation-induced tissue remodeling may be protective.

Place, publisher, year, edition, pages
eLife Sciences Publications Ltd, 2024. Vol. 12
Keywords [en]
PCOS, adipose tissue, genetics, genomics, human, medicine, methylation, mouse, proteomics, skeletal muscle, transcriptomics
National Category
Physiology Obstetrics, Gynecology and Reproductive Medicine Endocrinology and Diabetes Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Translational Medicine TRIM
Identifiers
URN: urn:nbn:se:his:diva-23514DOI: 10.7554/eLife.87592ISI: 001137149700001PubMedID: 38180081Scopus ID: 2-s2.0-85181630677OAI: oai:DiVA.org:his-23514DiVA, id: diva2:1824715
Funder
Swedish Research Council, 2020-02485Swedish Research Council, 2022-00550Novo Nordisk Foundation, NNF22OC0072904Swedish Research Council, 2020-01463Insamlingsstiftelsen Diabetes WellnessIngaBritt and Arne Lundberg’s Research Foundation
Note

CC BY 4.0 DEED

5 January 2024

Corresponding author: Anna Benrick, University of Gothenburg, Institute of Neuroscience and Physiology, Department of Physiology, Box 432, 405 30 Gothenburg, Sweden. Phone: +46 (0)709490148. E-mail: anna.benrick@gu.se

Funding: A.B. holds funding from the Swedish Research Council (2020-02485), E.SV. holds funding from the Swedish Research Council (2022-00550), the Novo Nordisk Foundation (NNF22OC0072904), and I.W.A. holds funding from the Swedish Research Council (2020-01463), Mary von Sydow Foundation, Diabetes Wellness Sverige, and EFSD//European Research Programme on ‘New Targets for Diabetes or Obesity-related Metabolic Diseases’ supported by MSD 2022, and J.N. holds funding from IngaBritt and Arne Lundberg Research Foundation.

Manuskript i medRxiv: DOI: 10.1101/2023.03.08.23286896

Available from: 2024-01-08 Created: 2024-01-08 Last updated: 2024-04-15Bibliographically approved

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