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Evidence that miR168a contributes to salinity tolerance of Brassica rapa L. via mediating melatonin biosynthesis
Department of Agriculture and Natural Resources, Higher Education Center of Eghlid, Iran.ORCID iD: 0000-0001-6449-305X
University of Skövde, School of Bioscience. University of Skövde, Systems Biology Research Environment. (Translationell bioinformatik, Translational Bioinformatics)ORCID iD: 0000-0003-1837-429X
Seed and Plant Improvement Research Department, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Shiraz, Iran.ORCID iD: 0000-0001-5590-1218
2022 (English)In: Physiologia Plantarum, ISSN 0031-9317, E-ISSN 1399-3054, Vol. 174, no 5, article id e13790Article in journal (Refereed) Published
Abstract [en]

Melatonin is a master regulator of diverse biological processes, including plant's abiotic stress responses and tolerance. Despite the extensive information on the role of melatonin in response to abiotic stress, how plants regulate endogenous melatonin content under stressful conditions remains largely unknown. In this study, we computationally mined Expressed Sequence Tag (EST) libraries of salinity-exposed Chinese cabbage (Brassica rapa) to identify the most reliable differentially expressed miRNA and its target gene(s). In light of these analyses, we found that miR168a potentially targets a key melatonin biosynthesis gene, namely O-METHYLTRANSFERASE 1 (OMT1). Accordingly, molecular and physiochemical evaluations were performed in a separate salinity experiment using contrasting B. rapa genotypes. Then, the association between B. rapa salinity tolerance and changes in measured molecular and physiochemical characteristics was determined. Results indicated that the expression profiles of miR168a and OMT1 significantly differed between B. rapa genotypes. Moreover, the expression profiles of miR168a and OMT1 significantly correlated with more melatonin content, robust antioxidant activities, and better ion homeostasis during salinity stress. Our results suggest that miR168a plausibly mediates melatonin biosynthesis, mainly through the OMT1 gene, under salinity conditions and thereby contributes to the salinity tolerance of B. rapa. To our knowledge, this is the first report on the role of miR168a and OMT1 in B. rapa salinity response.

Place, publisher, year, edition, pages
John Wiley & Sons, 2022. Vol. 174, no 5, article id e13790
Keywords [en]
physiological-responses, stress, accumulation, proline, targets
National Category
Biochemistry and Molecular Biology
Research subject
Bioinformatics
Identifiers
URN: urn:nbn:se:his:diva-21978DOI: 10.1111/ppl.13790ISI: 000864276800001PubMedID: 36169653Scopus ID: 2-s2.0-85140649007OAI: oai:DiVA.org:his-21978DiVA, id: diva2:1705045
Note

This research was supported by Department of Agriculture and Natural Resources, Higher Education Center of Eghlid, Eghlid, Iran.

Available from: 2022-10-20 Created: 2022-10-20 Last updated: 2023-01-17Bibliographically approved

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Lindlöf, Angelica

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