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Human intracardiac SSEA4+CD34 cells show features of cycling, immature cardiomyocytes and are distinct from Side Population and C-kit+CD45- cells
Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden ; Department of Clinical Chemistry, Region Västra Götaland, Sahlgrenska University Hospital, Gothenburg, Sweden.
Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden ; Department of Clinical Chemistry, Region Västra Götaland, Sahlgrenska University Hospital, Gothenburg, Sweden.
University of Skövde, School of Bioscience. University of Skövde, Systems Biology Research Environment. (Translationell bioinformatik, Translational Bioinformatics)ORCID iD: 0000-0001-9242-4852
Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden ; Department of Clinical Chemistry, Region Västra Götaland, Sahlgrenska University Hospital, Gothenburg, Sweden.
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2022 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 17, no 6, article id e0269985Article in journal (Refereed) Published
Abstract [en]

Cardiomyocyte proliferation has emerged as the main source of new cardiomyocytes in the adult. Progenitor cell populations may on the other hand contribute to the renewal of other cell types, including endothelial and smooth muscle cells. The phenotypes of immature cell populations in the adult human heart have not been extensively explored. We therefore investigated whether SSEA4+CD34- cells might constitute immature cycling cardiomyocytes in the adult failing and non-failing human heart. The phenotypes of Side Population (SP) and C-kit+CD45- progenitor cells were also analyzed. Biopsies from the four heart chambers were obtained from patients with end-stage heart failure as well as organ donors without chronic heart failure. Freshly dissociated cells underwent flow cytometric analysis and sorting. SSEA4+CD34- cells expressed high levels of cardiomyocyte, stem cell and proliferation markers. This pattern resembles that of cycling, immature, cardiomyocytes, which may be important in endogenous cardiac regeneration. SSEA4+CD34- cells isolated from failing hearts tended to express lower levels of cardiomyocyte markers as well as higher levels of stem cell markers. C-kit+CD45- and SP CD45- cells expressed high levels of endothelial and stem cell markers-corresponding to endothelial progenitor cells involved in endothelial renewal.

Place, publisher, year, edition, pages
PLOS , 2022. Vol. 17, no 6, article id e0269985
National Category
Cell and Molecular Biology
Research subject
Bioinformatics
Identifiers
URN: urn:nbn:se:his:diva-21252DOI: 10.1371/journal.pone.0269985ISI: 000843613300105PubMedID: 35709180Scopus ID: 2-s2.0-85132081811OAI: oai:DiVA.org:his-21252DiVA, id: diva2:1671433
Note

CC BY 4.0

Copyright:  © 2022 Sandstedt et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funding: This study was funded by grants from the Swedish Society of Medicine (JS, https://www.sls.se/); The Gothenburg Society of Medicine (MS,JS, https://goteborgslakaresallskap.se/); the Emelle Foundation (MS); the Heart-Lung Foundation (LMH, https://www.hjart-lungfonden.se/); ALF research grant from the Sahlgrenska University Hospital (JS, LMH, https://www.sahlgrenska.se/); grants from the foundations of the Sahlgrenska University Hospital (MS, JS, https://www.sahlgrenska.se/) and University of Skövde (JSy, https://www.his.se), by grants from the Swedish Knowledge Foundation (https://www.kks.se).There was no additional external fundingreceived for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Available from: 2022-06-17 Created: 2022-06-17 Last updated: 2023-08-23Bibliographically approved

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Ulfenborg, BenjaminSynnergren, Jane

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