Glucose being the primary source for cellular energy, maintains the glucose homeostasis. Glucose transporters (GLUTs) are carrier proteins for glucose uptake in cells. They are expressed in a tissue- and cell-specific way, and have unique kinetic and regulatory characteristics that illustrate their specific functional roles. This study focuses on uptake of glucose into the neuroendocrine cells by GLUTs and explores the distribution of the GLUTs and their trafficking. The use of PC12 cell line has provided a great deal of knowledge about the role of the proteins that underlie vesicle fusion. GLUT1 and GLUT2 were tagged with fluorescence proteins that is easily expressed in PC12 cells. Live cell imaging was done using total internal reflection microscope (TIRFM) after 24-48 hours of transfection and these data were analyzed using ImageJ. The distribution pattern highlights the prominent expression of GLUT1 compared to GLUT2. To understand the dynamics of these vesicles, the vesicle-plasma membrane interaction was explored. Three behavioral characteristics of the vesicles; docking, undocking and visits were observed based on the residence time of the vesicles. This experiment is the first to investigate the trafficking and fusion of GLUT1 and GLUT2 in PC12 cells, and to create a model system for studying neuronal glucose transporter regulation.