his.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
In vitro study of the antiproliferative properties of Digitoxin glycosides and Keytruda on BxPC-3 pancreatic cancer
University of Skövde, Health and Education.
2019 (English)Independent thesis Basic level (degree of Bachelor), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

In the early 1900s, cancer patients were often treated by inducing a bacterial infection that stimulated an immune response which lead to a spontaneous passive cancer regression. This discovery lead to today’s modern cancer immunotherapy – an approach in which medical interventions stimulate the body’s own immune system to fight cancer cells. Digitoxin glycosides, a sodium pump inhibitor used mainly to treat heart-related diseases has been reviewed in clinical trials for its anti-tumor like properties. Moreover, A new drug to aid in the war against progressive inoperable metastatic cancers was approved 2014 by FDA. Keytruda was the first monoclonal non-chimeric human IgG4 antibody drug to restore the immune response to activated T-cells by interfering with the tumours’ programmed death ligands (PD-L1) and (PD-L2). Cancer cells express an increased level of reactive oxygen species (ROS) that promote cancer cell proliferation and development. However, in too low or high levels, ROS promote oxidative damage in favour of anti-tumor properties. This study evaluated Digitoxin glycosides and Keytruda as potential anti-tumor therapies and any eventual synergic effects. Digitoxin, as mono-therapy, promoted apoptotic behaviour in pancreatic cancer cells when administrated in the mid- to high-end dosage range. Respectively, this study suggests a combination-therapy using a sub-physiological Keytruda-concentration together with a relatively high Digitoxin concentration produce a significant antiproliferative effect.

Place, publisher, year, edition, pages
2019. , p. 30
Keywords [en]
cytotoxic t lymphocytes, epidermal growth factor, fetal bovine serum, interferon gamma, immunoglobulin g, interleukin-1, monoclonal antibodies, natural killer cells, programmed death ligands, programmed death receptor, polymerase chain reaction, reactive oxygen species, tumor necrosis factor alpha, transforming growth factor β
National Category
Other Biological Topics Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:his:diva-17591OAI: oai:DiVA.org:his-17591DiVA, id: diva2:1346034
External cooperation
Johan Haux, medical consultant & cancer researcher
Subject / course
Biomedicine/Medical Science
Educational program
Biomedicine - Study Programme
Supervisors
Examiners
Available from: 2019-08-28 Created: 2019-08-27 Last updated: 2019-08-28Bibliographically approved

Open Access in DiVA

No full text in DiVA

Search in DiVA

By author/editor
Svensson, Andreas
By organisation
Health and Education
Other Biological TopicsBiomedical Laboratory Science/Technology

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 91 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf