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Evaluation of QuickFISH and maldi Sepsityper for identification of bacteria in bloodstream infection
University of Skövde, School of Bioscience. University of Skövde, The Systems Biology Research Centre. Department of Clinical Microbiology, Unilabs AB, Skövde, Sweden. (Infektionsbiologi, Infection Biology)ORCID iD: 0000-0001-7684-5702
University of Skövde, School of Bioscience. University of Skövde, The Systems Biology Research Centre. Department of Clinical Microbiology, Unilabs AB, Skövde, Sweden. (Infektionsbiologi, Infection Biology)
Department of Clinical Microbiology, Unilabs AB, Skövde, Sweden. (Infektionsbiologi, Infection Biology)
University of Skövde, School of Bioscience. University of Skövde, The Systems Biology Research Centre. Department of Clinical Microbiology, Unilabs AB, Skövde, Sweden. (Infektionsbiologi, Infection Biology)
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2019 (English)In: Infectious Diseases, ISSN 2374-4235, E-ISSN 2374-4243, Vol. 51, no 4, p. 249-258Article in journal (Refereed) Published
Abstract [en]

Background: Early detection of bacteria and their antibiotic susceptibility patterns are critical to guide therapeutic decision-making for optimal care of septic patients. The current gold standard, blood culturing followed by subculture on agar plates for subsequent identification, is too slow leading to excessive use of broad-spectrum antibiotic with harmful consequences for the patient and, in the long run, the public health. The aim of the present study was to assess the performance of two commercial assays, QuickFISH® (OpGen) and Maldi Sepsityper™ (Bruker Daltonics) for early and accurate identification of microorganisms directly from positive blood cultures.

Materials and methods: During two substudies of positive blood cultures, the two commercial assays were assessed against the routine method used at the clinical microbiology laboratory, Unilabs AB, at Skaraborg Hospital, Sweden.

Results: The Maldi Sepsityper™ assay enabled earlier microorganism identification. Using the cut-off for definite species identification according to the reference method (>2.0), sufficiently accurate species identification was achieved, but only among Gram-negative bacteria. The QuickFISH®assay was time-saving and showed high concordance with the reference method, 94.8% (95% CI 88.4–98.3), when the causative agent was covered by the QuickFISH® assay.

Conclusions: The use of the commercial assays may shorten the time to identification of causative agents in bloodstream infections and can be a good complement to the current clinical routine diagnostics. Nevertheless, the performance of the commercial assays is considerably affected by the characteristics of the causative agents.

Place, publisher, year, edition, pages
Taylor & Francis, 2019. Vol. 51, no 4, p. 249-258
Keywords [en]
MALDI-TOF MS analysis, QuickFISH®, sepsis diagnostics, blood culture, Maldi Sepsityper™
National Category
Other Medical Engineering Microbiology in the medical area Infectious Medicine Biomedical Laboratory Science/Technology
Research subject
Infection Biology
Identifiers
URN: urn:nbn:se:his:diva-16603DOI: 10.1080/23744235.2018.1554258ISI: 000465440800002PubMedID: 30729840Scopus ID: 2-s2.0-85061188564OAI: oai:DiVA.org:his-16603DiVA, id: diva2:1286666
Funder
Knowledge FoundationAvailable from: 2019-02-07 Created: 2019-02-07 Last updated: 2019-09-03Bibliographically approved

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Enroth, HelenaTilevik, DianaPernestig, Anna-Karin

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