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Structural and functional characterization of atPTR3, a stress-induced peptide transporter of Arabidopsis
Högskolan i Skövde, Institutionen för vård och natur.
Högskolan i Skövde, Institutionen för kommunikation och information.ORCID-id: 0000-0002-6549-086x
Högskolan i Skövde, Institutionen för vård och natur.
Högskolan i Skövde, Institutionen för vård och natur.
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2005 (Engelska)Ingår i: Journal of Molecular Modeling, ISSN 1610-2940, E-ISSN 0948-5023, Vol. 11, nr 3, s. 226-236Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A T-DNA tagged mutant line of Arabidopsis thaliana, produced with a promoter trap vector carrying a promoterless gus (uidA) as a reporter gene, showed GUS induction in response to mechanical wounding. Cloning of the chromosomal DNA flanking the T-DNA revealed that the insert had caused a knockout mutation in a PTR-type peptide transporter gene named At5g46050 in GenBank, here renamed AtPTR3. The gene and the deduced protein were characterized by molecular modelling and bioinformatics. Molecular modelling of the protein with fold recognition identified 12 transmembrane spanning regions and a large loop between the sixth and seventh helices. The structure of AtPTR3 resembled the other PTR-type transporters of plants and transporters in the major facilitator superfamily. Computer analysis of the AtPTR3 promoter suggested its expression in roots, leaves and seeds, complex hormonal regulation and induction by abiotic and biotic stresses. The computer-based hypotheses were tested experimentally by exposing the mutant plants to amino acids and several stress treatments. The AtPTR3 gene was induced by the amino acids histidine, leucine and phenylalanine in cotyledons and lower leaves, whereas a strong induction was obtained in the whole plant upon exposure to salt. Furthermore, the germination frequency of the mutant line was reduced on salt-containing media, suggesting that the AtPTR3 protein is involved in stress tolerance in seeds during germination.

Ort, förlag, år, upplaga, sidor
Springer, 2005. Vol. 11, nr 3, s. 226-236
Nyckelord [en]
Promoter trapping, Fold recognition, Peptide transporter, Wounding, Salt stress, Arabidopsis
Nationell ämneskategori
Botanik
Forskningsämne
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:his:diva-2381DOI: 10.1007/s00894-005-0257-6ISI: 000230142000006PubMedID: 15889294Scopus ID: 2-s2.0-22144460144OAI: oai:DiVA.org:his-2381DiVA, id: diva2:126872
Tillgänglig från: 2008-11-25 Skapad: 2008-11-25 Senast uppdaterad: 2020-01-29Bibliografiskt granskad

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Förlagets fulltextPubMedScopushttp://link.springer.com/article/10.1007%2Fs00894-005-0257-6

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Karim, SazzadLundh, DanHolmström, Kjell-OveMandal, Abul

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