Högskolan i Skövde

his.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Sepsis: Genotypic analysis of clinical Klebsiella spp. using next-generation sequencing
Högskolan i Skövde, Institutionen för biovetenskap. (Forskningscentrum i Systembiologi (Infektionsbiologi))
2018 (engelsk)Independent thesis Advanced level (degree of Master (Two Years)), 40 poäng / 60 hpOppgave
Abstract [en]

Sepsis is defined as a life-threatening organ dysfunction caused by a dysregulated host response system and can occur when the immune system over- or under- reacts to an infection. Klebsiella spp. has been found to be one of the leading causes of sepsis, and the increasing occurrence of antibiotic resistance observed has become a major concern in clinical care. To study the genome and increase knowledge of the biodiversity of K. pneumoniae, K. variicola, and K. oxytoca, bacterial isolates were collected from blood, urine, nasopharynx, and wounds of patients with suspected sepsis. Next-generation sequencing was performed, and the presence of antibiotic resistance genes and plasmids were studied. Furthermore, a prediction of traits for each phylogroup was performed and the results from whole-genome sequencing were compared to phenotypic results. Among the K. pneumoniae isolates obtained, almost half had been misidentified by standard phenotypic methods and were found to be K. variicola, K. quasipneumoniae, and K. quasivariicola. A significant difference in the number of antibiotic resistance genes were observed between K. pneumoniae and K. variicola compared to K. oxytoca, however no significant difference was observed between K. pneumoniae and K. variicola, suggesting the underestimated pathogenicity of K. variicola. A genetic agreement was observed between the type of beta-lactamase harboured and presence or absence of nitrogen-fixation genes to the phylogroup, providing a way of species identification. Further studies should be conducted on the pathogenicity and virulence of K. variicola and K. quasipneumoniae to avoid misidentification, find organism-specific treatments, and narrow down the antibiotic prescription.

sted, utgiver, år, opplag, sider
2018. , s. 50
Emneord [en]
Sepsis, Klebsiella, systems biology, next-generation sequencing, NGS
HSV kategori
Identifikatorer
URN: urn:nbn:se:his:diva-15842OAI: oai:DiVA.org:his-15842DiVA, id: diva2:1225335
Eksternt samarbeid
Unilabs, Skövde (Klinisk mikrobiologi och klinisk bakteriologi); SciLifeLab, Solna; Skaraborgs sjukhus, Skövde (Infektionsmottagningen)
Fag / kurs
Systems Biology
Utdanningsprogram
Tumor Biology - Master’s Programme
Presentation
2018-05-30, G106, Högskolevägen 1, Skövde, 08:15 (engelsk)
Veileder
Examiner
Prosjekter
Biodiversitet vid SepsisTilgjengelig fra: 2018-06-28 Laget: 2018-06-26 Sist oppdatert: 2018-06-28bibliografisk kontrollert

Open Access i DiVA

Master thesis(2297 kB)537 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 2297 kBChecksum SHA-512
fb56c0b170d3d346cf7649a3aae473499b0a76a7636d3bb954e52d387385a45dd6e0d2c9ef2d0b5b40998620e5caa71a14ff0b3c3e92b983538e4a333d056a0e
Type fulltextMimetype application/pdf

Søk i DiVA

Av forfatter/redaktør
Saxenborn, Patricia
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 537 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

urn-nbn

Altmetric

urn-nbn
Totalt: 586 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf