his.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Localization of AtHOG1 and AtHOG2 in Arabidopsis plants at the tissue and subcellular levels
Högskolan i Skövde, Institutionen för vård och natur.
2010 (Engelska)Självständigt arbete på avancerad nivå (magisterexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
Abstract [en]

Plant hormones are responsible for plant growth and adaptation to the environment. Among them the most important are cytokinins. Plants undergo gene silencing processes called homology-dependent gene silencing processes. In Arabidopsis there are two homology-dependent gene silencing genes that were chosen for further study, namely AtHOG1 and AtHOG2. Transgenic plants were generated previously with ten different constructs containing AtHOG1 or AtHOG2 genes and were used in this study. Some of the constructs had GFP attached so that the protein expressed could be visualised in a confocal microscope. Transgenic plants generated were T1 and T2 generations. Their DNA was extracted from leaves. By means of PCR transgenic plants were identified. There were 147 samples. Among them there were 39 positiveswith BAR primers and 32 positives with construct specific primers. The localisation of the HOG2 protein was observed in a confocal microscope. Seeds used were T3 generation and were obtained from the lab. HOG2 protein was found to be localised in cell membrane, root tip and chloroplasts.

Ort, förlag, år, upplaga, sidor
2010. , s. 25
Nyckelord [en]
transgenic Arabidopsis, transgenic plants, HOG1 gene
Nationell ämneskategori
Biokemi och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:his:diva-4446OAI: oai:DiVA.org:his-4446DiVA, id: diva2:359859
Uppsök
bio-/geovetenskap
Handledare
Examinatorer
Tillgänglig från: 2010-11-04 Skapad: 2010-11-01 Senast uppdaterad: 2010-11-04Bibliografiskt granskad

Open Access i DiVA

fulltext(1422 kB)1012 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 1422 kBChecksumma SHA-512
b13d7017eec264e5f7a0cee439701660680423746607f952348c03889e168c5dfea45a19054f1180e50db758309d57e17ae9ca690498c37923d90bfd109de42b
Typ fulltextMimetyp application/pdf

Av organisationen
Institutionen för vård och natur
Biokemi och molekylärbiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 1012 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 308 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf