Högskolan i Skövde

his.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Dissecting the population dynamics of antibiotic-resistant and antibiotic-sensitive clones of uropathogenic Escherichia coli (UPEC) isolated from patients with urinary tract infection
Högskolan i Skövde, Institutionen för biovetenskap.
2023 (Engelska)Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
Abstract [en]

The most common type of bacterial infection is urinary tract infection (UTI). More than 80% of uncomplicated UTIs are caused by uropathogenic Escherichia coli (UPEC). This illness is not only limited to the urinary tract; it may also entail a systemic infection, which makes matters worse. UTIs are treated with various antibiotics, however antimicrobial resistance (AMR) is now causing more fatalities around the world. One of the primary causes of AMR is extended-spectrum beta-lactamase producers (ESBL). Moreover, AMR is linked to virulence and antibiotic-resistance genes(ARGs). The objective of this study was to describe the population of E. coli producing ESBL and sensitive E. coli from our collection of strains from clinical urine cultures and blood from 137 patients with UTI and blood stream infection and study their phenotypic and genotypic characteristics. To carry out this project antibiotic susceptibility test, rapid typing of bacterial isolates, and whole genome sequencing was done. UPEC strains were mostly sensitive to meropenem, imipenem, tigecycline, nitrofurantoin, fosfomycin, and mecillinam, whereas 38.2% of strains were ESBL-positive and 48.9% of strains exhibited multidrug resistance. The intra-host diversity in ESBL-positive samples was greater than in non-ESBL samples. Therefore, antibiotic resistance was caused by several types of E. coli instead of a single clone which might be the reason for recurrent UTI. The majority of the isolates, according to genomic analysis, belong to phylogroup B2, with ST131 being the most common sequence type and fimH30 being the most closely linked virulence gene to UPEC. One of the concerning findings in UPEC analysis is the prevalence of antimicrobial resistance genes within the bacterial strains. These antimicrobial resistance genes, such as blaCTX-M-15, blaCTX-M-27, and blaEC-5, are responsible for conferring resistance to beta-lactam antibiotics and are commonly found in UPEC strains. Moreover, genes associated with resistance to sulfonamides, tetracycline, trimethoprim, aminoglycosides, and even colistin, a last-resort antibiotic for treating life-threatening conditions, have also been identified. The presence of these antimicrobial resistance genes highlights the need for vigilance in managing antibiotic use to prevent the further spread of resistance.

Ort, förlag, år, upplaga, sidor
2023. , s. 30
Nationell ämneskategori
Bioinformatik och systembiologi
Identifikatorer
URN: urn:nbn:se:his:diva-22726OAI: oai:DiVA.org:his-22726DiVA, id: diva2:1769289
Ämne / kurs
Systembiologi
Utbildningsprogram
Infektionsbiologi - masterprogram, 120 hp
Handledare
Examinatorer
Tillgänglig från: 2023-06-16 Skapad: 2023-06-16 Senast uppdaterad: 2023-06-16Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Av organisationen
Institutionen för biovetenskap
Bioinformatik och systembiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 208 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf