Högskolan i Skövde

his.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Investigating possible differential expression level of hsa-miR-708-5p in Neuroblastoma
Högskolan i Skövde, Institutionen för biovetenskap.
2022 (Engelska)Självständigt arbete på grundnivå (kandidatexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
Abstract [en]

Neuroblastoma (NB) is one of the most common extracranial cancers found in children under the age of five. The cause of NB is not well understood, about 2% of the cases have been linked to rare germline mutations in the anaplastic lymphoma kinase (ALK) gene. However, NB is thought to be mainly caused by genetic mutation at the early stages of development. Clinically, NB can be grouped into three risk groups: low, intermediate and high-risk disease. The survival rate of patients with high-risk NB is less than 50% of the diagnosed cases. Survival rates emphasizes the necessity for future NB diagnostic therapy. One potential study area is miRNA, studies have demonstrated both prognostic and predictive usefulness to therapies. MiRNA is a single-stranded RNA that is 18-24 nucleotides long. Its function is to regulate numerous cellular activities, and to act as tumor suppressors or oncogenes. Genetic anomalies such as MYCN amplification and 11q deletion cause NB by disrupting the expression patterns of certain miRNAs. In this experiment the miRNA, hsa-miR-708-5p, was examined in three genetically diverse NB cell lines; NB69 without MYCN amplification and 11q deletion, SKNBE with MYCN amplification, and Kelly with a chromosome 11q deletion, the cell lines were used to see if the expression levels of hsa-miR-708-5p differed. The expression level of hsa-miR-708-5p, was assessed using qPCR; variation in gene expression was identified between the cell lines. Therefore, miR-708-5p could be a viable option when looking at gene expression of hsa-miR-708-5p for future diagnostic or prognostic in NB.

Ort, förlag, år, upplaga, sidor
2022. , s. 42
Nationell ämneskategori
Medicinsk biovetenskap
Identifikatorer
URN: urn:nbn:se:his:diva-22164OAI: oai:DiVA.org:his-22164DiVA, id: diva2:1722410
Ämne / kurs
Biovetenskap
Utbildningsprogram
Biovetenskap - Molekylär biodesign
Handledare
Examinatorer
Tillgänglig från: 2022-12-29 Skapad: 2022-12-29 Senast uppdaterad: 2022-12-29Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Av organisationen
Institutionen för biovetenskap
Medicinsk biovetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 283 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf