Högskolan i Skövde

his.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Quantification of the expression of hsa-mir-34c-5p in neuroblastoma cells
Högskolan i Skövde, Institutionen för biovetenskap.
2022 (Engelska)Självständigt arbete på grundnivå (kandidatexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
Abstract [en]

Neuroblastomas are the most common extra-cranial abnormal growths in immature nerve tissue within the sympathetic nervous system, in children ages 0-5. It is a heterogeneous malignancy, often emerging in and around adrenal glands, caused by rapid growth and division of nerve cells. From a clinical perspective it is difficult to treat as it has spontaneous regression, rapid progression and therapy resistance. MicroRNAs (miRNA) are small, single stranded RNA, 18-25 nucleotides long and act as human gene expression regulators which are deregulated in Neuroblastoma. The expression of miRNAs can be analysed to determine the progressive stage at which the neuroblastoma is, and the best course of treatment can be decided. The aim of this study was to analyse the expression of miRNA hsa-miR-34c-5p, in three different cell lines each having different genetic characteristics, NB69 without MYCN amplification and 11 q deletion, SN-BE with MYCN amplification and Kelly with 11q deletion. As hsa-miR-34c-5p has been found to have tumour suppressive capabilities in Neuroblastoma cell lines. The cell lines were cultured, the total RNA was isolated, Reverse Transcription was done to convert small RNA to cDNA and qPCR was run and Cq values were obtained. The miRNA expression was determined using qPCR and Livak´s method, however, no statistical difference was found in the expression of hsa-miR-34c-5p between the Neuroblastoma cell lines. These results are only a steppingstone in future research of miRNA and Neuroblastoma. 

Ort, förlag, år, upplaga, sidor
2022. , s. 25
Nationell ämneskategori
Medicinsk biovetenskap
Identifikatorer
URN: urn:nbn:se:his:diva-22139OAI: oai:DiVA.org:his-22139DiVA, id: diva2:1720649
Ämne / kurs
Biovetenskap
Utbildningsprogram
Biovetenskap - Molekylär biodesign
Handledare
Examinatorer
Tillgänglig från: 2022-12-20 Skapad: 2022-12-20 Senast uppdaterad: 2022-12-20Bibliografiskt granskad

Open Access i DiVA

fulltext(1236 kB)141 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 1236 kBChecksumma SHA-512
b28969f9711a4e81ee2666117a1b07acab10bfbaa495b1edeb1e8abbf5b864ced6874b02783051beee76bd171c939b3ecfae6178bc8b04bb9a23c122403f873e
Typ fulltextMimetyp application/pdf

Av organisationen
Institutionen för biovetenskap
Medicinsk biovetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 141 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 176 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf