Affective touch is found in various social interactions, i.e., romantic couples or between family members. It was disclosed that this type of touch could diminish the HPA axis activity and thus, cortisol levels, mainly when one is exposed to stress. This study aimed to discover a potential association between polymorphisms important for the HPA axis and cortisol levels when affective touch is given. Statistical analyses were performed on SNPs within relevant genes (rs41423247 from NR3C1 gene, rs1360780 in FKBP5 gene, rs53576 in OXTR gene, rs6295 within HTR1A gene and 5-HTTLPR in SLC6A4 gene) and basal/final cortisol values of women touched either by their partner or a stranger. Relevant associations were found only between the rs6295 (HTR1A gene), rs1360780 (FKBP5 gene) and cortisol values of women receiving first touch from their partner. Consequently, the carriers of GG genotype in rs6295 and carriers of CT/TT in rs1360780 had lower cortisol values. Interestingly, the G-allele of rs6295 was formerly related to a weakened cortisol response, whereas the T-allele of rs1360780 was associated with a lower recovery of the cortisol levels. A newly added SNP, rs242939 in CRHR1 gene was genotyped performing the PCR-RFLP method, but the statistical analysis did not show any association with the cortisol. Overall, this project has successfully revealed a connection between two of the analyzed polymorphisms and the cortisol values of the women touched first by their partner. This demonstrated that affective touch has positive effects on the cortisol release, hence on the activity of HPA axis.