Vagal afferent neurons (VAN) possess terminals in numerous organs of the body and intervene different sensory modalities. Studies suggests that these neurons have varying functions in both males and females in relation to feeding responses, obesity and more. Furthermore, studies also demonstrated there were sex differences in mice VAN as the deletion of VAN leptin receptors showed instinctive feeding response and deposition of excess adipose tissue. Sequel to this, studies have shown that there are a few functional differences in the VAN with sex – specific vagal communication and for that reason, the hypothesis of the project was that there are also molecular differences between male and female mice and the aim was to identify the differences using existing single – cell RNA - sequencing (scRNA-seq) datasets from the articles of Kupari et al. and Bai et al. respectively. The authors both investigated the vagal sensory neurons of mice using the scRNA-seq. Three datasets from the afore mentioned papers were analyzed with the aid of different Bioinformatic tools. Firstly, genes mapping to the Y chromosomes were identified and the sum of their expression was used to assign sex (male or female) to individual cell samples in the datasets, and the classification was also validated using the Xist gene. Secondly, the seurat pipeline which is the suitable tool for analyzing scRNA-seq datasets was employed to identify differentially expressed genes (DEGs) between VAN from male and female mice and in each cluster of the VAN. The results were then analyzed and visualized using principal component analysis (PCA), t-Distributed Stochastic Neighbour Embedding (tSNE), volcano and violin plots. The study identified six genes namely: Gadd45a, 1810037I17Rik, Tmem50b, Eif2s3x, 1810041L15Rik and Nek7 as potential sex difference marker genes. The six genes identified were not only linked to the sex-linked chromosomes but to other chromosomes in the mice with expressions in different organs of the body particularly the brain, ovary, and testis. More so, few of the genes suggest positive and negative impact in diseases like down syndrome and cancer particularly ovarian cancer which is common to females.