Högskolan i Skövde

his.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Comparison of extraction method for miRNA as a biomarker for the diagnosis of sepsis: Future diagnostics of sepsis
Högskolan i Skövde, Institutionen för biovetenskap.
2020 (Engelska)Självständigt arbete på grundnivå (kandidatexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
Abstract [en]

Sepsis is a severe condition caused by a dysregulated host response to an infection that may leadto organ failure and death. Early diagnostic to be able to provide correct treatment is crucial forsurvival. Performing a blood culture is the most common way in the diagnostic of sepsis, a timeconsumingprocess with many false negative results. MicroRNA has been suggested as apotential biomarker for sepsis due to the stability of the microRNA and the possibility todiagnose more complex diseases. Blood was donated from self-assessed healthy individuals andthe plasma was used for extractions of microRNA. Two different isolation kits were used inextractions with a starting plasma volume of 100 μl and 200 μl to determine which of the kitsthat provide the highest amount of miRNA concentration with the highest quality, the kits werethen compared to each other. The two kits used were miRNeasy Serum/Plasma Advanced kit(Qiagen) and Total RNA Purification kit (Norgen). The result from the extractions were analyzedin SPSS and showed a statistically significant difference of microRNA concentration. ThemiRNeasy kit had a higher miRNA concentration in the extractions than the Norgen kit while thequality of the extractions did not show any significant difference between the two kits. ThemiRNeasy kit show a possibility of providing extractions with a high microRNA concentrationusing both a starting plasma volume of 100 μl and 200 μl and could be a possible kit to use infurther extractions in the research of sepsis.

Ort, förlag, år, upplaga, sidor
2020. , s. 23
Nationell ämneskategori
Medicinsk biovetenskap
Identifikatorer
URN: urn:nbn:se:his:diva-18936OAI: oai:DiVA.org:his-18936DiVA, id: diva2:1459578
Ämne / kurs
Biovetenskap
Utbildningsprogram
Biovetenskap - Molekylär biodesign
Handledare
Examinatorer
Tillgänglig från: 2020-08-20 Skapad: 2020-08-20 Senast uppdaterad: 2020-08-20Bibliografiskt granskad

Open Access i DiVA

fulltext(841 kB)826 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 841 kBChecksumma SHA-512
fb9badb30e00db7a39aea9c5ed3398221a6f5c6d87e839f41c7c707f632071f9dad2e0f3a12a7d786404706d76addb2e1a1b25e77f27100ff05ca0efa7d9c7c9
Typ fulltextMimetyp application/pdf

Av organisationen
Institutionen för biovetenskap
Medicinsk biovetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 827 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 509 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf