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A PCOS-like Drosophila Melanogaster model
Högskolan i Skövde, Institutionen för hälsa och lärande. Högskolan i Skövde, Institutionen för hälsovetenskaper.
2019 (Engelska)Självständigt arbete på grundnivå (kandidatexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
Abstract [en]

Polycystic ovary syndrome (PCOS) is a female endocrine disorder defined by high androgen levels and presence of polycystic ovaries. PCOS is characterized by menstrual irregularities, anovulation, infertility, hyperandrogenism, insulin resistance, abdominal obesity, chronic inflammation and increased hair growth. The diagnosis is based on 2003/2004 Rotterdam criteria, which is based on the presence of the following phenotypes: anovulation, clinical and biochemical marks of hyperandrogenism and polycystic ovarian morphology. Theoretical causes could be genetical, environmental or maternal imprinting. Drosophila Melanogaster, a model used broadly in disease research, could bear promising insights to this syndrome. Besides having a lifecycle characterized by a 12 days metamorphism, these species of flies have the ecdysone (steroid) hormone, similar to the human testosterone and the body systems similar to those of the human body. This laboratory work involved the development of a PCOS-like drosophila fly model through exposure to 10mg/ml of testosterone after 24 hours of starvation. Data collection comprised measurements of weight and length, anovulation, triglyceride quantification and RT-qPCR for quantification of inflammatory and PCOS-related genes. Results showed significant differences in response to physical stress among the four groups of flies. Variation in weight and length values, as well as in fecundity, triglyceride assay and relative expression levels were also observed. Although the expression levels of inflammatory and PCOS- related genes were not significantly affected, homeostasis was clearly affected by metabolic disturbances. These observations lead to the conclusion that further experiments should be done in order to establish a more comprehensive definition of the syndrome.

 

 

Ort, förlag, år, upplaga, sidor
2019. , s. 39
Nyckelord [en]
polycystic, ovary, syndrome, testosterone
Nyckelord [la]
drosophila, melanogaster
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:his:diva-17845OAI: oai:DiVA.org:his-17845DiVA, id: diva2:1367314
Ämne / kurs
Biomedicin/medicinsk vetenskap
Utbildningsprogram
Biomedicinprogrammet
Handledare
Examinatorer
Tillgänglig från: 2020-10-26 Skapad: 2019-11-02 Senast uppdaterad: 2020-10-26Bibliografiskt granskad

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A PCOS-like Drosophila Melanogaster model(1122 kB)438 nedladdningar
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