his.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
The Effects of Probiotics on High Sugar-Induced Type 2 Diabetes Mellitus Symptoms in Drosophila melanogaster
Högskolan i Skövde, Institutionen för hälsa och lärande.
2019 (Engelska)Självständigt arbete på grundnivå (kandidatexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
Abstract [en]

Background: Type 2 diabetes mellitus is a metabolic disorder characterized by the rise of fasting plasma glucose from its normal range (≥125mg/dl). It is marked by insufficient production of insulin from pancreatic β-cells as a result of failed compensation due to insulin resistance. Several treatments are available for the disorder, which mainly focus on improving the sensitivity of insulin in different body tissues. Recently, probiotics were suggested as candidate treatments for type 2 diabetes and for extending lifespan as well. This experiment aims to investigate such claims using Drosophila melanogaster as a disease model.

 

Results: Other than the observed low average weights in treated larva samples, probiotics did not show any other significant results in affecting the length, glucose, glycogen, and trehalose levels (One-Way ANOVA and Kruskal-Wallis, p>0.05). Real-time PCR was only carried out once. Thus, no statistical tests were reliable enough to analyse the data obtained. The longevity study, on the other hand, did show significance (Log-rank (Mantel-Cox) test and Gehan-Breslow-Wilcoxon test, p<0.0001), as the probiotic Bifidobacterium lactis extended the lifespan of adult flies feeding on a high sugar diet significantly when compared to the control ones feeding on only high sugar diet without probiotics.

 

Conclusion: Except for weight measurements, none of the other results was reliable enough to make a concrete conclusion on whether the treatments indeed worked in reversing type 2 diabetes symptoms or not. Real-time PCR results did show some effects of some of the treatments at different developmental stages. However, unless Real-time PCR is repeated at least once using the same protocol, no deduction can be made. Additionally, the data obtained hint that the dosage used (0.025 g) was too high for larvae and adult flies and might have caused malnutrition by blocking their midgut and decreasing food absorption. Hence, false significant or non-significant results were acquired instead.

 

Further studies are required using a much lower probiotic dosage if Drosophila is used as a disease model. Although, other models such as mice or rats are recommended in this case, in order to reach a solid conclusion about the effectiveness of probiotics in treating type 2 diabetes mellitus. Baring these thoughts in mind and based on the results of this experiment, the null hypothesis indicating that there is no significant relationship between the use of probiotics and reversing type 2 diabetes mellitus symptoms is therefore accepted.

Ort, förlag, år, upplaga, sidor
2019. , s. 87
Nyckelord [en]
Diabetes, Biomedicine, PEPCK, FOXO, Fbp, Hex-t1, Zw, Drosophila, Melanogaster, Longevity, Probiotics
Nationell ämneskategori
Endokrinologi och diabetes
Identifikatorer
URN: urn:nbn:se:his:diva-17817OAI: oai:DiVA.org:his-17817DiVA, id: diva2:1366003
Ämne / kurs
Biomedicin/medicinsk vetenskap
Utbildningsprogram
Biomedicinprogrammet
Handledare
Examinatorer
Tillgänglig från: 2019-12-06 Skapad: 2019-10-28 Senast uppdaterad: 2019-12-06Bibliografiskt granskad

Open Access i DiVA

fulltext(3273 kB)31 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 3273 kBChecksumma SHA-512
02d42094a7624ac3f78d990f16a2530f3acf0a44382981e719003fba3c5d9cb3f33b896404ea65e4eba4a165dc261fb4f0ce97115cc950261ee8f16c553639e8
Typ fulltextMimetyp application/pdf
fulltext(3005 kB)10 nedladdningar
Filinformation
Filnamn FULLTEXT02.pdfFilstorlek 3005 kBChecksumma SHA-512
266d6738cbdb0be2538ebc4bac56d71c0f0db0b8616c3e729ffacc81ca7960f990048e95c349be3d1528a9581bdba3fc30cac0f2e719fe871dc5e4250472c49f
Typ fulltextMimetyp application/pdf

Av organisationen
Institutionen för hälsa och lärande
Endokrinologi och diabetes

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 41 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 150 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf