his.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Detection of exosomal mirna from different volumes of biofluids as biomarkers for the diagnosis of sepsis: Future diagnostics of sepsis
Högskolan i Skövde, Institutionen för biovetenskap.
2019 (Engelska)Självständigt arbete på grundnivå (kandidatexamen), 20 poäng / 30 hpStudentuppsats (Examensarbete)
Abstract [en]

Sepsis, a life-threatening condition which results from a dysregulation of host response to infection and leads to multiple organ dysfunction, is a cause for great concern. The current gold standard of detection – Blood culturing – is a highly time-consuming process and so, research has proposed the use of biomarkers. Current biomarkers, C-reactive protein and Procalcitonin, though good indicators, individually show certain limitations with respect to the specificity and sensitivity. Hence, as a step forward from singleplex biomarkers, the development of a multi-marker panel was suggested. For this purpose, the use of microRNAs (miRNAs) were employed to serve as potential biomarkers for the detection of sepsis. The aim of this study was to determine whether a higher concentration of miRNA would be obtained from a larger volume of plasma as well as to see if the miRNA present in blood can be used for the diagnosis of sepsis. Extractions were carried out using the QIAGEN exoRNeasy Plasma: Midi & Maxi Kits from plasma and Norgen’s Total RNA Purification Kit from blood. The samples were analysed and quantified using the Qubit® microRNA assay kit & Qubit® 3.0 Fluorometer and the NanoDrop™ 2000 Spectrophotometer. Statistical analysis of the results revealed that there was a significant difference between miRNA concentrations in the two volumes of plasma analysed. Based on the accurate Qubit measurements and readings, it was concluded that a larger volume of plasma, does yield a higher concentration of miRNA. In addition, it was also established that the miRNA detected in blood, could be used as probable biomarkers for the diagnosis of sepsis.

Ort, förlag, år, upplaga, sidor
2019. , s. 36
Nyckelord [en]
MicroRNA, Biomarker, Rapid diagnosis, Sepsis, Plasma, Nanodrop, Qubit, Concentration, Purity
Nationell ämneskategori
Medicin och hälsovetenskap
Identifikatorer
URN: urn:nbn:se:his:diva-17592OAI: oai:DiVA.org:his-17592DiVA, id: diva2:1346031
Ämne / kurs
Molekylärbiologi
Handledare
Examinatorer
Tillgänglig från: 2019-08-27 Skapad: 2019-08-27 Senast uppdaterad: 2019-08-27Bibliografiskt granskad

Open Access i DiVA

a19animo_Bachelors Thesis(756 kB)57 nedladdningar
Filinformation
Filnamn FULLTEXT02.pdfFilstorlek 756 kBChecksumma SHA-512
fcfbc5430e6935af8521a25c92ec3a49ce926bec6a6db83745eaa5aa69e21b5809f7009dd05bd62cdce8ef24cfa437a36eef1f53a7c0e8e339533fa52ce74784
Typ fulltextMimetyp application/pdf

Sök vidare i DiVA

Av författaren/redaktören
Monteiro, Anita-Ann
Av organisationen
Institutionen för biovetenskap
Medicin och hälsovetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 57 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 310 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf