his.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Deciphering the ontogeny of unmutated and mutated subsets of Chronic Lymphocytic Leukemia
Högskolan i Skövde, Institutionen för biovetenskap.
2019 (Engelska)Självständigt arbete på avancerad nivå (masterexamen), 30 poäng / 45 hpStudentuppsats (Examensarbete)
Abstract [en]

Chronic Lymphocytic Leukemia (CLL) is a type of cancer that affects the B cells of the immune system causing problems in the process of producing antibodies. It can be sorted into mutated and unmutated CLL based on the percentage of somatic mutations in the Immunoglobulin Heavy chain Variable region (IgHV). The B cells of healthy individuals can be sorted into three groups; CD27dull memory B cells (MBCs), CD27bright MBCs and naïve B cells. The hypothesis for the project was that the unmutated CLL subset originates from CD27dull MBCs and the mutated CLL subset originates from CD27bright MBCs. RNA-sequencing data from healthy individuals were acquired from a collaboration partner in Rome and CLL-patients were collected from public datasets available online. Several bioinformatic tools were used to analyze the data. First, the quality of the data files was checked, then adapter sequence from the sequencing process and low-quality bases were removed (trimming). Good quality of the files was confirmed after the trimming. Secondly, these files were mapped against the human reference genome (GRCh38/hg38) for alignment, then the resulted data was used to check for genes that showed differential expression between the different groups. Results were analyzed and visualized using Venn diagrams, Principal Component Analysis (PCA) and heatmap plots and random forest. A list of 85 genes was generated based on the different comparisons and was used in one PCA plot that showed clear separation between the different groups. The SWAP70 gene was analyzed for single nucleotide polymorphisms (SNPs). The study concluded five genes that could be used as biomarkers for CLL and the diagnosis of its subtypes where some of them were discussed in previous studies. Also, the mutated CLL subset showed a similar behavior to the healthy individuals and this could validate the original hypothesis and justifies the better disease prognosis for this subtype.

Ort, förlag, år, upplaga, sidor
2019. , s. 33
Nyckelord [en]
CLL, NGS, mutated CLL, unmutated CLL, CD27 bright, CD27 dull, memory B cell, RNA-sequencing
Nationell ämneskategori
Bioinformatik och systembiologi Immunologi
Identifikatorer
URN: urn:nbn:se:his:diva-17286OAI: oai:DiVA.org:his-17286DiVA, id: diva2:1330079
Externt samarbete
University of Gothenburg
Ämne / kurs
Systembiologi
Utbildningsprogram
Molekylär bioteknik - masterprogram
Handledare
Examinatorer
Tillgänglig från: 2019-06-26 Skapad: 2019-06-25 Senast uppdaterad: 2019-06-26Bibliografiskt granskad

Open Access i DiVA

fulltext(1790 kB)33 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 1790 kBChecksumma SHA-512
6350ab9d49a21d0c3c1c287871e29db186cd6b1b206812ded103eea077d32f06483d5f9c3eb4343a3fdca689b143a702fd2ad8d836cf14b8857d22e296dec25c
Typ fulltextMimetyp application/pdf

Av organisationen
Institutionen för biovetenskap
Bioinformatik och systembiologiImmunologi

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 33 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

urn-nbn

Altmetricpoäng

urn-nbn
Totalt: 171 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf