his.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Differences in microRNA expression during tumor development in the transition and peripheral zones of the prostate
Örebro University / Örebro University Hospital.
Örebro University / Örebro University Hospital.
Örebro University / Örebro University Hospital.
Örebro University / Örebro University Hospital.
Visa övriga samt affilieringar
2013 (Engelska)Ingår i: BMC Cancer, ISSN 1471-2407, E-ISSN 1471-2407, Vol. 13, artikel-id 362Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: The prostate is divided into three glandular zones, the peripheral zone (PZ), the transition zone (TZ), and the central zone. Most prostate tumors arise in the peripheral zone (70-75%) and in the transition zone (20-25%) while only 10% arise in the central zone. The aim of this study was to investigate if differences in miRNA expression could be a possible explanation for the difference in propensity of tumors in the zones of the prostate. Methods: Patients with prostate cancer were included in the study if they had a tumor with Gleason grade 3 in the PZ, the TZ, or both (n=16). Normal prostate tissue was collected from men undergoing cystoprostatectomy (n=20). The expression of 667 unique miRNAs was investigated using TaqMan low density arrays for miRNAs. Student's t-test was used in order to identify differentially expressed miRNAs, followed by hierarchical clustering and principal component analysis (PCA) to study the separation of the tissues. The ADtree algorithm was used to identify markers for classification of tissues and a cross-validation procedure was used to test the generality of the identified miRNA-based classifiers. Results: The t-tests revealed that the major differences in miRNA expression are found between normal and malignant tissues. Hierarchical clustering and PCA based on differentially expressed miRNAs between normal and malignant tissues showed perfect separation between samples, while the corresponding analyses based on differentially expressed miRNAs between the two zones showed several misplaced samples. A classification and cross-validation procedure confirmed these results and several potential miRNA markers were identified. Conclusions: The results of this study indicate that the major differences in the transcription program are those arising during tumor development, rather than during normal tissue development. In addition, tumors arising in the TZ have more unique differentially expressed miRNAs compared to the PZ. The results also indicate that separate miRNA expression signatures for diagnosis might be needed for tumors arising in the different zones. MicroRNA signatures that are specific for PZ and TZ tumors could also lead to more accurate prognoses, since tumors arising in the PZ tend to be more aggressive than tumors arising in the TZ.

Ort, förlag, år, upplaga, sidor
BioMed Central, 2013. Vol. 13, artikel-id 362
Nyckelord [en]
MiRNA expression, Prostate cancer, Prostate zones, microRNA, microRNA 127 3p, microrna 154, microRNA 15a, microRNA 15b, microRNA 181c, microRNA 216b, microRNA 22, microRNA 27b, microRNA 337 3p, microrna 379, microRNA 424, microRNA 433, microrna 494, microRNA 495, microRNA 543, phosphatidylinositol 3, 4, 5 trisphosphate 3 phosphatase, transforming growth factor beta, unclassified drug, adult, aged, article, cancer grading, cell cycle arrest, cell cycle regulation, central zone, clinical article, controlled study, gene expression, gene function, gene targeting, human, human tissue, male, pathogenesis, peripheral zone, prostate, tissue differentiation, transition zone
Nationell ämneskategori
Naturvetenskap
Forskningsämne
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:his:diva-8710DOI: 10.1186/1471-2407-13-362ISI: 000322598200001PubMedID: 23890084Scopus ID: 2-s2.0-84880941948OAI: oai:DiVA.org:his-8710DiVA, id: diva2:683140
Tillgänglig från: 2014-01-02 Skapad: 2014-01-02 Senast uppdaterad: 2017-12-06Bibliografiskt granskad

Open Access i DiVA

fulltext(2745 kB)457 nedladdningar
Filinformation
Filnamn FULLTEXT01.pdfFilstorlek 2745 kBChecksumma SHA-512
776fe3d4ea31dcea6790ed80b038c131d22e470298e34d90c72fe476a5b876261ef39ac52a8f9815b043f9fd5183d6165725f8e07787688a8664e42f3c61d500
Typ fulltextMimetyp application/pdf

Övriga länkar

Förlagets fulltextPubMedScopus

Personposter BETA

Klinga-Levan, KarinOlsson, Björn

Sök vidare i DiVA

Av författaren/redaktören
Klinga-Levan, KarinOlsson, Björn
Av organisationen
Institutionen för vård och naturForskningscentrum för Systembiologi
I samma tidskrift
BMC Cancer
Naturvetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar
Totalt: 457 nedladdningar
Antalet nedladdningar är summan av nedladdningar för alla fulltexter. Det kan inkludera t.ex tidigare versioner som nu inte längre är tillgängliga.

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 1212 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf