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Verification of microRNA expression in human endometrial adenocarcinoma
Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi. (Biomedicinsk genetik, Biomedical genetics / Infektionsbiologi, Infection Biology)
Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi. (Biomedicinsk genetik, Biomedical genetics)
Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi. (Bioinformatik, Bioinformatics)ORCID-id: 0000-0001-6254-4335
Högskolan i Skövde, Institutionen för biovetenskap. Högskolan i Skövde, Forskningscentrum för Systembiologi. (Bioinformatik, Bioinformatics)ORCID-id: 0000-0001-8962-0860
2016 (engelsk)Inngår i: BMC Cancer, ISSN 1471-2407, E-ISSN 1471-2407, Vol. 16, nr 1, artikkel-id 261Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background: MicroRNAs are small non-coding RNAs that have been implicated in tumor initiation and progression. In a previous study we identified 138 miRNAs as differentially expressed in endometrial adenocarcinoma compared to normal tissues. One of these miRNAs was miRNA-34a, which regulates several genes involved in the Notch pathway, which is frequently altered in endometrial cancer. The aims of this study were to verify the differential expression of a subset of miRNAs and to scrutinize the regulatory role of mir-34a on the target genes NOTCH1 and DLL1. Methods: Twenty-five miRNAs that were previously identified as differentially expressed were subjected to further analysis using qPCR. To investigate the regulation of NOTCH1 and DLL1 by mir-34a, we designed gain- and loss-of-function experiments in Ishikawa and HEK293 cell lines by transfection with a synthetic mir-34a mimic and a mir-34a inhibitor. Results: Of the 25 validated miRNAs, seven were down-regulated and 18 were up-regulated compared to normal endometrium, which was fully consistent with our previous findings. In addition, the up-regulation of mir-34a led to a significant decrease in mRNA levels of NOTCH1 and DLL1, while down-regulation led to a significant increase in mRNA levels of these two genes. Conclusions: We verified both up-regulated and down-regulated miRNAs in the tumor samples, indicating various roles of microRNAs during tumor development. Mir-34a functions as a regulator by decreasing the expression of NOTCH1 and DLL1. Our study is the first to identify a correlation between mir-34a and its target genes NOTCH1 and DLL1 in endometrial adenocarcinoma.

sted, utgiver, år, opplag, sider
BioMed Central (BMC), 2016. Vol. 16, nr 1, artikkel-id 261
Emneord [en]
Endometrial adenocarcinoma, microRNA, mir-34a, Target genes
HSV kategori
Forskningsprogram
Biomedicinsk genetik; Bioinformatik; Infektionsbiologi
Identifikatorer
URN: urn:nbn:se:his:diva-10890DOI: 10.1186/s12885-016-2296-zISI: 000373329900001PubMedID: 27039384Scopus ID: 2-s2.0-84962003924OAI: oai:DiVA.org:his-10890DiVA, id: diva2:810722
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CC BY 4.0

Tilgjengelig fra: 2015-05-05 Laget: 2015-05-05 Sist oppdatert: 2023-09-21bibliografisk kontrollert
Inngår i avhandling
1. MicroRNA expression profiling in endometrial adenocarcinoma
Åpne denne publikasjonen i ny fane eller vindu >>MicroRNA expression profiling in endometrial adenocarcinoma
2015 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
sted, utgiver, år, opplag, sider
Örebro: Örebro university, 2015. s. 53
Serie
Örebro Studies in Medicine, ISSN 1652-4063 ; 118
Emneord
Endometrial cancer, microRNA, BDII rat model, normalization, endogenous controls
HSV kategori
Forskningsprogram
Medicin
Identifikatorer
urn:nbn:se:his:diva-10886 (URN)9789175290638 (ISBN)
Disputas
2015-03-27, Portalen, Högskolan i Skövde, Högskolevägen, 09:00 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2015-05-04 Laget: 2015-05-04 Sist oppdatert: 2023-05-02bibliografisk kontrollert

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Jurcevic, SanjaKlinga-Levan, KarinOlsson, BjörnEjeskär, Katarina

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