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RNAseq Analysis of Gastric Bacteria in Helicobacter pylori-Associated Carcinogenesis
Högskolan i Skövde, Institutionen för biovetenskap.
2014 (engelsk)Independent thesis Advanced level (degree of Master (One Year)), 20 poäng / 30 hpOppgave
Abstract [en]

Helicobacter pylori infects more than half of the world's population, and is known to be involved in several diseases including gastric cancer. Its close interactions with the stomach and host immune system serves as a good model to study the co-adaptation and co-evolution of the organisms in the stomach micro-environment. In this project, we utilized RNA-seq and data analysis tools to investigate differentially expressed genes by H. pylori in patients at different stages of early gastric cancer development. We also investigated the abundance and diversity of bacterial genera other than H. pylori, and looked for correlations with H. pylori presence and number. For differential gene expression of H. pylori, one gene was differentially expressed between samples of corpus atrophy without metaplasia vs. samples of antrum gastritis, and eight genes were found to be differentially expressed between samples of corpus atrophy with metaplasia vs. samples with pan-gastritis. When samples were clustered into different groups based on the expression data, 52 genes (shared or unique to the specific comparison groups) were found to be differentially expressed, but no apparent patterns were observed that could be explained by medical or sample collection data. For bacterial diversity and abundances, we found several genera colonizing the stomach, of which some have been previously identified. While most of these bacteria colonize regardless of the presence of H. pylori, the abundance of three genera, Wolinella, Campylobacter, and Veillonella, seem to be correlated with the presence of H. pylori.

sted, utgiver, år, opplag, sider
2014. , s. 33
HSV kategori
Identifikatorer
URN: urn:nbn:se:his:diva-9937OAI: oai:DiVA.org:his-9937DiVA, id: diva2:745501
Fag / kurs
Bioinformatics
Utdanningsprogram
Bioinformatics - Master’s Programme
Presentation
2014-06-09, Skövde, 11:00 (engelsk)
Veileder
Examiner
Tilgjengelig fra: 2014-09-11 Laget: 2014-09-10 Sist oppdatert: 2014-09-11bibliografisk kontrollert

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RNAseq Analysis of Gastric Bacteria in Helicobacter pylori-Associated Carcinogenesis(948 kB)287 nedlastinger
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