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In Situ PLA image analysis using Blob Finder tool for evaluation of protein interaction
Högskolan i Skövde, Institutionen för vård och natur. (Prof.Pierre Aman's Group)
2010 (engelsk)Independent thesis Advanced level (degree of Master (One Year)), 10 poäng / 15 hpOppgave
Abstract [en]

In recent biomedical research, images are generated at the high rates by using modern fluorescence microscopy. There are different image analysis software that can be used to perform a wide array of useful image analysis measurement. The identification of protein interaction is important for the detail study of localization and quantification of the expression. The main aim of the project was to evaluate the interactions of FUS/Plectin proteins in fibrosarcoma cells using the Blobfinder tool. The FUS/Plectin interaction localization was showed using In Situ Proximity ligation based assay (PLA) and these protein interactions were quantified by the Blobfinder tool. The obtained quantitative results from the tool were statistically analyzed by Graphpad prism software and Microsoft Excel. PLA results showed the localization of the FUS/Plectin interaction in the cytoplasm and on the border of nucleus. The Blobfinder tool obtained quantitative results which suggested that there were more interactions in the cytoplasm as compare to the nucleus. The histogram analysis also suggests that the frequency of interactions were higher in the cytoplasm when compared to nucleus in majority of cells. The obtained results from the Blobfinder tool and In situ PLA are in agreement with the results obtained from Immunoprecipitation pull down assay which shows FUS/Plectin interactions.

sted, utgiver, år, opplag, sider
2010. , s. 20
HSV kategori
Identifikatorer
URN: urn:nbn:se:his:diva-4541OAI: oai:DiVA.org:his-4541DiVA, id: diva2:383428
Fag / kurs
Bioinformatics
Utdanningsprogram
Bioinformatics - Master’s Programme
Presentation
2010-09-15, Portalen room, University of Skövde, Sweden, Skövde, 14:00 (engelsk)
Uppsök
Medicine
Veileder
Examiner
Tilgjengelig fra: 2011-09-15 Laget: 2011-01-04 Sist oppdatert: 2011-09-15bibliografisk kontrollert

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