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Localization of AtHOG1 and AtHOG2 in Arabidopsis plants at the tissue and subcellular levels
Högskolan i Skövde, Institutionen för vård och natur.
2010 (engelsk)Independent thesis Advanced level (degree of Master (One Year)), 20 poäng / 30 hpOppgave
Abstract [en]

Plant hormones are responsible for plant growth and adaptation to the environment. Among them the most important are cytokinins. Plants undergo gene silencing processes called homology-dependent gene silencing processes. In Arabidopsis there are two homology-dependent gene silencing genes that were chosen for further study, namely AtHOG1 and AtHOG2. Transgenic plants were generated previously with ten different constructs containing AtHOG1 or AtHOG2 genes and were used in this study. Some of the constructs had GFP attached so that the protein expressed could be visualised in a confocal microscope. Transgenic plants generated were T1 and T2 generations. Their DNA was extracted from leaves. By means of PCR transgenic plants were identified. There were 147 samples. Among them there were 39 positiveswith BAR primers and 32 positives with construct specific primers. The localisation of the HOG2 protein was observed in a confocal microscope. Seeds used were T3 generation and were obtained from the lab. HOG2 protein was found to be localised in cell membrane, root tip and chloroplasts.

sted, utgiver, år, opplag, sider
2010. , s. 25
Emneord [en]
transgenic Arabidopsis, transgenic plants, HOG1 gene
HSV kategori
Identifikatorer
URN: urn:nbn:se:his:diva-4446OAI: oai:DiVA.org:his-4446DiVA, id: diva2:359859
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Life Earth Science
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Tilgjengelig fra: 2010-11-04 Laget: 2010-11-01 Sist oppdatert: 2010-11-04bibliografisk kontrollert

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