his.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
The Pseudomonas aeruginosa Quorum-Sensing Molecule N-3-(Oxododecanoyl)-L-Homoserine Lactone Inhibits T-Cell Differentiation and Cytokine Production by a Mechanism Involving an Early Step in T-Cell Activation
Sch. of Biotech. and Biomol. Sci., University of New South Wales, Sydney, NSW 2052, Australia.
Högskolan i Skövde, Institutionen för vård och natur.
Sch. of Biotech. and Biomol. Sci., University of New South Wales, Sydney, NSW 2052, Australia.
Högskolan i Skövde, Institutionen för vård och natur.
Vise andre og tillknytning
2005 (engelsk)Inngår i: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 73, nr 3, s. 1648-1655Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The Pseudomonas aeruginosa quorum-sensing molecule N-3-(oxododecanoyl)-L-homoserine lactone (OdDHL) has been reported to have immunomodulatory activity in several systems, although the mechanism of that activity remains to be fully characterized. We demonstrate here, using a defined in vitro model of antigen responses by T-cell receptor (TCR)-transgenic mouse splenic CD4 T cells, that the effect of OdDHL on activation and cytokine production is complete within 4 h of antigen or mitogen stimulation and does not depend on the insertion of OdDHL in the cell membrane, despite a previous report that immunosuppression by homoserine lactones required a minimum acyl chain length of 11 carbons (S. R. Chhabra, C. Harty, D. S. W. Hooi, M. Daykin, B. W. Bycroft, P. Williams, and D. Pritchard, J. Med. Chem. 46:97-104, 2003). We also demonstrate that while OdDHL can have toxic effects on nonlymphoid leukocytes, it does not induce significant cell death in T cells at the concentrations (≤10 µM) used in these experiments. In addition, we show that primary and secondary antigen-specific cytokine responses are equally susceptible to inhibition by OdDHL and that the compound inhibits the differentiation of both Th1 and Th2 cells. However, the precise balance of cytokine production by CD4 T cells stimulated in the presence of OdDHL varies with both the antigen concentration and its affinity for the transgenic TCR. Thus, conflicting reports of the nature of the immunosuppression by OdDHL may be due in part to the differences in antigen affinity and concentration in different models.

sted, utgiver, år, opplag, sider
American Society for Microbiology , 2005. Vol. 73, nr 3, s. 1648-1655
HSV kategori
Forskningsprogram
Naturvetenskap
Identifikatorer
URN: urn:nbn:se:his:diva-1574DOI: 10.1128/IAI.73.3.1648-1655.2005ISI: 000227373300043PubMedID: 15731065Scopus ID: 2-s2.0-15544384442OAI: oai:DiVA.org:his-1574DiVA, id: diva2:31850
Tilgjengelig fra: 2007-07-23 Laget: 2007-07-23 Sist oppdatert: 2017-12-12bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMedScopus

Personposter BETA

Jansson, AndreasNilsson, Patric

Søk i DiVA

Av forfatter/redaktør
Jansson, AndreasNilsson, Patric
Av organisasjonen
I samme tidsskrift
Infection and Immunity

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 901 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf