his.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Effects of 1,25(OH)2D3 on Smad2 Activity in PC-3 Prostate Cancer Cells
Högskolan i Skövde, Institutionen för vård och natur.
2009 (engelsk)Student paper other, 5 poäng / 7,5 hpOppgave
Abstract [en]

The vitamin D metabolite 1,25(OH)2D3 has long been known to inhibit growth of prostate cancer cells and this mainly through a VDR-mediated pathway controlling target gene expression, resulting in cell cycle arrest, apoptosis and differentiation. Another major way inwhich 1,25(OH)2D3 inhibits cell growth in prostate cancer is via membrane-initiated steroid signalling, which triggers activation of signal cascades upon steroid binding to a receptor complex, leading to induction of genes regulating cell growth, proliferation and apoptosis. The main prostate cancer inhibiting membrane-initiated route is the TGFβ signalling pathway, elicited by the protein TGFβ. Another important protein downstream in this cascade is Smad2. In this study the early effects of 1,25(OH)2D3 on activated Smad2 levels in PC-3 prostate cancer cells were examined. PC-3 cells were incubated for 5, 10, 30 and 60 minutes as well as 24 and 40 hours both together with 1,25(OH)2D3 of the concentrations 10-10 and 107 M and without. An ELISA assay scanning for activated Smad2 was then performed on supernatants from both treated and untreated cells. This is a follow-up to an earlier study which examined the influence of 1,25(OH)2D3 on TGFβ levels using the same doses and similar time points and which found that 1,25(OH)2D3 initially lowered the level of active TGFβ, then increased it. The results of this study showed a statistically insignificant, time delayed 1,25(OH)2D3 mediated induction of the same pattern in the levels of active Smad2.

sted, utgiver, år, opplag, sider
2009. , s. 12
Emneord [en]
prostate cancer; PC-3; vitamin D; 1, 25(OH)2D3; TGFβ; Smad2; Smad3
HSV kategori
Identifikatorer
URN: urn:nbn:se:his:diva-3807OAI: oai:DiVA.org:his-3807DiVA, id: diva2:304579
Presentation
(engelsk)
Uppsök
Medicine
Veileder
Examiner
Prosjekter
Project Work in Biomedicine, Advanced Level, 7.5 ECTSTilgjengelig fra: 2010-03-23 Laget: 2010-03-18 Sist oppdatert: 2010-03-23bibliografisk kontrollert

Open Access i DiVA

fulltekst(514 kB)174 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 514 kBChecksum SHA-512
c9a9e8f8dd545ed44af98f06c280562d0855f50076a4107f88fd84a4fa91f14c788dd2ac31e9f2f304b15644700f7fa3679c5d2c9f834b75937615e91f8a452a
Type fulltextMimetype application/pdf

Søk i DiVA

Av forfatter/redaktør
Stahel, Anette
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 174 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

urn-nbn

Altmetric

urn-nbn
Totalt: 199 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf