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Design and Development of a Database for the Classification of Corynebacterium glutamicum Genes, Proteins, Mutants and Experimental Protocols
Högskolan i Skövde, Institutionen för kommunikation och information.
2006 (engelsk)Independent thesis Advanced level (degree of Master (One Year)), 20 poäng / 30 hpOppgave
Abstract [en]

Coryneform bacteria are largely distributed in nature and are rod like, aerobic soil bacteria capable of growing on a variety of sugars and organic acids. Corynebacterium glutamicum is a nonpathogenic species of Coryneform bacteria used for industrial production of amino acids. There are three main publicly available genome annotations, Cg, Cgl and NCgl for C. glutamicum. All these three annotations have different numbers of protein coding genes and varying numbers of overlaps of similar genes. The original data is only available in text files. In this format of genome data, it was not easy to search and compare the data among different annotations and it was impossible to make an extensive multidimensional customized formal search against different protein parameters. Comparison of all genome annotations for construction deletion, over-expression mutants, graphical representation of genome information, such as gene locations, neighboring genes, orientation (direct or complementary strand), overlapping genes, gene lengths, graphical output for structure function relation by comparison of predicted trans-membrane domains (TMD) and functional protein domains protein motifs was not possible when data is inconsistent and redundant on various publicly available biological database servers. There was therefore a need for a system of managing the data for mutants and experimental setups. In spite of the fact that the genome sequence is known, until now no databank providing such a complete set of information has been available. We solved these problems by developing a standalone relational database software application covering data processing, protein-DNA sequence extraction and

management of lab data. The result of the study is an application named, CORYNEBASE, which is a software that meets our aims and objectives.

sted, utgiver, år, opplag, sider
Skövde: Institutionen för kommunikation och information , 2006. , s. 24
HSV kategori
Identifikatorer
URN: urn:nbn:se:his:diva-23OAI: oai:DiVA.org:his-23DiVA, id: diva2:2584
Presentation
(engelsk)
Uppsök
Physics, Chemistry, Mathematics
Veileder
Examiner
Tilgjengelig fra: 2006-11-06 Laget: 2006-11-06 Sist oppdatert: 2018-01-13

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95019f248b3912ce4104017339d567db31b0d6c4893c377625e6ddbe55d4ae199a965870
Type fulltextMimetype application/pdf

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