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Quantification of the expression of hsa-mir-34c-5p in neuroblastoma cells
Högskolan i Skövde, Institutionen för biovetenskap.
2022 (engelsk)Independent thesis Basic level (degree of Bachelor), 20 poäng / 30 hpOppgave
Abstract [en]

Neuroblastomas are the most common extra-cranial abnormal growths in immature nerve tissue within the sympathetic nervous system, in children ages 0-5. It is a heterogeneous malignancy, often emerging in and around adrenal glands, caused by rapid growth and division of nerve cells. From a clinical perspective it is difficult to treat as it has spontaneous regression, rapid progression and therapy resistance. MicroRNAs (miRNA) are small, single stranded RNA, 18-25 nucleotides long and act as human gene expression regulators which are deregulated in Neuroblastoma. The expression of miRNAs can be analysed to determine the progressive stage at which the neuroblastoma is, and the best course of treatment can be decided. The aim of this study was to analyse the expression of miRNA hsa-miR-34c-5p, in three different cell lines each having different genetic characteristics, NB69 without MYCN amplification and 11 q deletion, SN-BE with MYCN amplification and Kelly with 11q deletion. As hsa-miR-34c-5p has been found to have tumour suppressive capabilities in Neuroblastoma cell lines. The cell lines were cultured, the total RNA was isolated, Reverse Transcription was done to convert small RNA to cDNA and qPCR was run and Cq values were obtained. The miRNA expression was determined using qPCR and Livak´s method, however, no statistical difference was found in the expression of hsa-miR-34c-5p between the Neuroblastoma cell lines. These results are only a steppingstone in future research of miRNA and Neuroblastoma. 

sted, utgiver, år, opplag, sider
2022. , s. 25
HSV kategori
Identifikatorer
URN: urn:nbn:se:his:diva-22139OAI: oai:DiVA.org:his-22139DiVA, id: diva2:1720649
Fag / kurs
Bioscience
Utdanningsprogram
Bioscience - Molecular Biodesign
Veileder
Examiner
Tilgjengelig fra: 2022-12-20 Laget: 2022-12-20 Sist oppdatert: 2022-12-20bibliografisk kontrollert

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