Measuring Biodiversity is an important phenomenon to serve best to our ecology and also keep environment sound. Variety of life on different levels, like an ecosystem, life forms on a site, landscape collectively known as Biodiversity. Species richness and evenness combine measures as Biodiversity. Separate formula, index, equation are widely using to measure Biodiversity in each level. Swedish Environmental Protection Agency aimed to establish an index that consists of landscape functionality and landscape heterogeneity. For landscape functionality assessment, there BBCI (Biotope biodiversity Capacity index) is going to use. High BBCI indicates a high biodiversity for each biotope. However, empirically estimate species richness how much matched with BBCI that not been evaluated. The aim of this paper to see the relationship between empirical estimated Biodiversity and BBCI. A relationship between Shannon diversity index and BBCI also ran to see the matches between them. Collect the empirical data from selected 15 landscapes using Artportalen.se and sort the data for further calculation. Results showed that there was a strong positive relationship between empirical estimated Biodiversity and BBCI. Again Shannon diversity index and BBCI also demonstrated a positive correlation between them. It showed BBCI could explain 60%-69% of species richness data and 17%-22% of Shannon diversity index. It indicates the acceptance of theoretical study of measure Biodiversity.