Högskolan i Skövde

his.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Transcriptomic analysis on freshwater mussels for identification of potential biomarkers to monitor water ecosystems
Högskolan i Skövde, Institutionen för biovetenskap.
2020 (engelsk)Independent thesis Advanced level (degree of Master (One Year)), 20 poäng / 30 hpOppgave
Abstract [en]

Stress-specific expression of cellular proteins in responses to exogenous exposure and resulting physiological alteration provides important insight into the field of ecological research. Due to its habitat, feeding, lifestyle and physiologic properties, mussel has become an important indicative measure of aquatic environment pollution in order to assess effect of these pollution in aquatic life. In order to minimize the threats imposed on the aquatic ecosystem and advancement of sustainable lifestyle for human, recent ecological studies are more concern about monitoring different bioindicative properties. In this study, two widely distributed freshwater bivalve mussel species Anodonta anatina and Unio tumidus was used to conduct comparative study on the transcriptome of these species in order to identify and quantify the expressed transcripts on both species and investigate their biomarker properties in mussels for monitoring heavy metal or toxic exposure. mRNA was isolated and converted to cDNA through reverse transcription PCR. Quality and quantity assessments of purity, fragment size and concentration was performed. Each cDNA sample was barcoded and amplified for cDNA library preparation and nanopore sequencing. Basic bioinformatics tools were used to identify the transcripts for transcriptomic analysis. The findings shows some common mitochondrial and ribosomal transcripts along with a wide range of conserved and abundant transcript variants in mussels with important biomarker properties. Some of the transcripts exhibits expression in multiple samples suggesting characteristic bioindicator properties. Also in this study, a pipeline for transcriptomic analysis was generated and critical steps in the procedure were identified and discussed.

sted, utgiver, år, opplag, sider
2020. , s. 28
HSV kategori
Identifikatorer
URN: urn:nbn:se:his:diva-19285OAI: oai:DiVA.org:his-19285DiVA, id: diva2:1505457
Eksternt samarbeid
Knowledge foundation
Fag / kurs
Bioscience
Veileder
Examiner
Tilgjengelig fra: 2020-12-01 Laget: 2020-12-01 Sist oppdatert: 2020-12-01bibliografisk kontrollert

Open Access i DiVA

fulltext(1669 kB)329 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 1669 kBChecksum SHA-512
35289ce1a6a10d12586436af395b7dfbc2dcbbe2893fc82f20c2af4c28b16998ef781b4ca0febcc73f362f59169773ccbeff4f144007efad7902bfecc607012d
Type fulltextMimetype application/pdf

Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 329 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

urn-nbn

Altmetric

urn-nbn
Totalt: 1080 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf