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Comparison of extraction method for miRNA as a biomarker for the diagnosis of sepsis: Future diagnostics of sepsis
Högskolan i Skövde, Institutionen för biovetenskap.
2020 (engelsk)Independent thesis Basic level (degree of Bachelor), 20 poäng / 30 hpOppgave
Abstract [en]

Sepsis is a severe condition caused by a dysregulated host response to an infection that may leadto organ failure and death. Early diagnostic to be able to provide correct treatment is crucial forsurvival. Performing a blood culture is the most common way in the diagnostic of sepsis, a timeconsumingprocess with many false negative results. MicroRNA has been suggested as apotential biomarker for sepsis due to the stability of the microRNA and the possibility todiagnose more complex diseases. Blood was donated from self-assessed healthy individuals andthe plasma was used for extractions of microRNA. Two different isolation kits were used inextractions with a starting plasma volume of 100 μl and 200 μl to determine which of the kitsthat provide the highest amount of miRNA concentration with the highest quality, the kits werethen compared to each other. The two kits used were miRNeasy Serum/Plasma Advanced kit(Qiagen) and Total RNA Purification kit (Norgen). The result from the extractions were analyzedin SPSS and showed a statistically significant difference of microRNA concentration. ThemiRNeasy kit had a higher miRNA concentration in the extractions than the Norgen kit while thequality of the extractions did not show any significant difference between the two kits. ThemiRNeasy kit show a possibility of providing extractions with a high microRNA concentrationusing both a starting plasma volume of 100 μl and 200 μl and could be a possible kit to use infurther extractions in the research of sepsis.

sted, utgiver, år, opplag, sider
2020. , s. 23
HSV kategori
Identifikatorer
URN: urn:nbn:se:his:diva-18936OAI: oai:DiVA.org:his-18936DiVA, id: diva2:1459578
Fag / kurs
Bioscience
Utdanningsprogram
Bioscience - Molecular Biodesign
Veileder
Examiner
Tilgjengelig fra: 2020-08-20 Laget: 2020-08-20 Sist oppdatert: 2020-08-20bibliografisk kontrollert

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