Högskolan i Skövde

his.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Dynamic peptide-folding mediated biofunctionalization and modulation of hydrogels for 4D bioprinting
Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, Sweden.
Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, Sweden.
Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, Sweden.
Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, Sweden.
Vise andre og tillknytning
2020 (engelsk)Inngår i: Biofabrication, ISSN 1758-5082, E-ISSN 1758-5090, Vol. 12, nr 3, artikkel-id 035031Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Hydrogels are used in a wide range of biomedical applications, including three-dimensional (3D) cell culture, cell therapy and bioprinting. To enable processing using advanced additive fabrication techniques and to mimic the dynamic nature of the extracellular matrix (ECM), the properties of the hydrogels must be possible to tailor and change over time with high precision. The design of hydrogels that are both structurally and functionally dynamic, while providing necessary mechanical support is challenging using conventional synthesis techniques. Here, we show a modular and 3D printable hydrogel system that combines a robust but tunable covalent bioorthogonal cross-linking strategy with specific peptide-folding mediated interactions for dynamic modulation of cross-linking and functionalization. The hyaluronan-based hydrogels were covalently cross-linked by strain-promoted alkyne-azide cycloaddition using multi-arm poly(ethylene glycol). In addition, a de novo designed helix-loop-helix peptide was conjugated to the hyaluronan backbone to enable specific peptide-folding modulation of cross-linking density and kinetics, and hydrogel functionality. An array of complementary peptides with different functionalities was developed and used as a toolbox for supramolecular tuning of cell-hydrogel interactions and for controlling enzyme-mediated biomineralization processes. The modular peptide system enabled dynamic modifications of the properties of 3D printed structures, demonstrating a novel route for design of more sophisticated bioinks for four-dimensional bioprinting. © 2020 The Author(s). Published by IOP Publishing Ltd.

sted, utgiver, år, opplag, sider
Institute of Physics Publishing (IOPP), 2020. Vol. 12, nr 3, artikkel-id 035031
Emneord [en]
biomineralization, bioprinting, folding, hydrogel, hyaluronan, peptide, Cell culture, Hyaluronic acid, Hydrogels, Medical applications, Modulation, Peptides, Polyethylene glycols, Biomedical applications, Biomineralization process, Conventional synthesis, Covalently cross-linked, Cross-linking density, Dynamic modifications, Extracellular matrices, Three-dimensional (3d) cell culture, 3D printers
HSV kategori
Forskningsprogram
Bioinformatik
Identifikatorer
URN: urn:nbn:se:his:diva-18906DOI: 10.1088/1758-5090/ab9490ISI: 000548339700001PubMedID: 32428894Scopus ID: 2-s2.0-85087529583OAI: oai:DiVA.org:his-18906DiVA, id: diva2:1457739
Merknad

CC BY 4.0

Correspondence Address: Aili, D.; Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, Sweden; email: daniel.aili@liu.se

Tilgjengelig fra: 2020-08-12 Laget: 2020-08-12 Sist oppdatert: 2023-09-21bibliografisk kontrollert

Open Access i DiVA

fulltext(4275 kB)241 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 4275 kBChecksum SHA-512
252747c1b847a98b049c71cc2d9655e1af355263209e12fa2eac24e7e14beb7af1084cd7f34a6a5dd96c5d033e7602e1ab992af53d932876cc59c7d4252fa797
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekstPubMedScopus

Person

Christoffersson, Jonas

Søk i DiVA

Av forfatter/redaktør
Christoffersson, Jonas
Av organisasjonen
I samme tidsskrift
Biofabrication

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 241 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 442 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • apa-cv
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf