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Construction of a Fusion Gene: to anchor a truncated version of the inflammatory receptor NLRP3 to the cell membrane
Högskolan i Skövde, Institutionen för biovetenskap.
2019 (engelsk)Independent thesis Basic level (degree of Bachelor), 20 poäng / 30 hpOppgave
Abstract [en]

Inflammasomes are a group of protein complex that regulate inflammation throughcomplex signal transduction, although their specific mechanisms and structures have notbeen fully described. As the protein that kickstarts assembly of a type of inflammasome,NLRP3 is a key regulator of inflammation and may play a relevant role in the developmentof inflammatory diseases. In this project it has been attempted to perform a Gene Fusionbetween a segment of NLRP3 and regions of Toll-Like Receptor 4 by means of overlapextensionPCR, a technique that employs hybrid primers to create an overlap between bothsequences that can be filled by a polymerase, causing them to merge. Results suggest GeneFusion was successful, however cloning and expression of the construct have not beenachieved so far. If expressed as a fusion protein, the added transmembrane domain willanchor two domains of NLRP3 to the membrane, allowing more precise study of thecomposition and functionality of the inflammasome. Removal of the terminal domain ofNLRP3 will help determine its implication and relevance in the assembly process of theprotein complex.

sted, utgiver, år, opplag, sider
2019. , s. 25
Emneord [en]
NLRP3, Inflammation, Fusion gene, PCR, Fusion protein
HSV kategori
Identifikatorer
URN: urn:nbn:se:his:diva-17573OAI: oai:DiVA.org:his-17573DiVA, id: diva2:1345386
Fag / kurs
Bioscience
Utdanningsprogram
Bioscience - Molecular Biodesign
Veileder
Examiner
Tilgjengelig fra: 2019-08-26 Laget: 2019-08-23 Sist oppdatert: 2019-08-26bibliografisk kontrollert

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