Sepsis is defined by the body's inflammatory response to infection. It is a rapidly progressive condition in which a systemic response to an infection mediated by endogenous mediators, can result in a broad inflammatory reaction in organs distant from the actual injury and end in endorgan dysfunction or even failure. As of a 2020 study, sepsis remains the leading cause of death inhospitals. Currently, a single test for the diagnosis of sepsis does not exist, therefore hospitals rely on testing a combination of vital parameters and observing common symptoms associated with sepsis. However, these methods are often time-consuming, making them fatal for the patients. The University of Skövde is looking into combining different biomarkers into one multimarker panel. Their current research focuses on miRNA as a potential biomarker for sepsis. Despite being a promising field, miRNA extraction from plasma has its difficulties. The study extracted miRNA using miRNeasy Serum/Plasma Advanced Kit (Qiagen) kit and QIAcube (Qiagen) to determine whether manual or semi-automatic extraction is better in terms of time, quality, and quantity. The second aim of the study was to quantify and identify miR-Seps 3 using the RT-qPCR method. The two methods of extraction quality, quantity, and time did not have a significant difference. TheRT-qPCR method was able to amplify miR-Seps 3, though further research is needed on whether the miRNA can be a possible candidate for multimarker panel.