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Nahar, Noor
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Publications (10 of 16) Show all publications
Nahar, N., Rahman, A., Ghosh, S., Nawani, N. & Mandal, A. (2017). Functional studies of AtACR2 gene putatively involved in accumulation, reduction and/or sequestration of arsenic species in plants. Biologia (Bratislava), 72(5), 520-526
Open this publication in new window or tab >>Functional studies of AtACR2 gene putatively involved in accumulation, reduction and/or sequestration of arsenic species in plants
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2017 (English)In: Biologia (Bratislava), ISSN 0006-3088, E-ISSN 1336-9563, Vol. 72, no 5, p. 520-526Article in journal (Refereed) Published
Abstract [en]

Food-based exposure to arsenic is a human carcinogen and can severely impact human health resulting in many cancerous diseases and various neurological and vascular disorders. This project is a part of our attempts to develop new varieties of crops for avoiding arsenic contaminated foods. For this purpose, we have previously identified four key genes, and molecular functions of two of these, AtACR2 and AtPCSl, have been studied based on both in silico and in vivo experiments. In the present study, a T-DNA tagged mutant, (SALK-143282C with mutation in AtACR2 gene) of Arabidopsis thaliana was studied for further verification of the function of AtACR2 gene. Semi-quantitative RT-PCR analyses revealed that this mutant exhibits a significantly reduced expression of the AtACR2 gene. When exposed to 100 μM of arsenate (AsV) for three weeks, the mutant plants accumulated arsenic approximately three times higher (778 μg/g d. wt.) than that observed in the control plants (235 μg/g d. wt.). In contrast, when the plants were exposed to 100 μM of arsenite (AsIII), no significant difference in arsenic accumulation was observed between the control and the mutant plants (535 μg/g d. wt. and 498 μg/g d. wt., respectively). Also, when arsenate and arsenite was measured separately either in shoots or roots, significant differences in accumulation of these substances were observed between the mutant and the control plants. These results suggest that AtACR2 gene is involved not only in accumulation of arsenic in plants, but also in conversion of arsenate to arsenite inside the plant cells. © 2017 Institute of Molecular Biology, Slovak Academy of Sciences.

Place, publisher, year, edition, pages
Springer, 2017
Keywords
Arabidopsis thaliana, arsenate reductase 2 gene, arsenic accumulation, arsenic speciation, IC-ICP-DRC-MS, RT-PCR
National Category
Biochemistry and Molecular Biology Botany
Research subject
Biotechnology
Identifiers
urn:nbn:se:his:diva-14272 (URN)10.1515/biolog-2017-0062 (DOI)000404241300006 ()2-s2.0-85021444188 (Scopus ID)
Available from: 2017-10-30 Created: 2017-10-30 Last updated: 2019-11-25Bibliographically approved
Rahman, A., Nahar, N., Nawani, N. N. & Mandal, A. (2017). Investigation on Arsenic-Accumulating and Arsenic-Transforming Bacteria for Potential Use in the Bioremediation of Arsenics. In: Surajit Das, Hirak Ranjan Dash (Ed.), Handbook of Metal-Microbe Interactions and Bioremediation: (pp. 509-519). Boca Raton, FL: CRC Press
Open this publication in new window or tab >>Investigation on Arsenic-Accumulating and Arsenic-Transforming Bacteria for Potential Use in the Bioremediation of Arsenics
2017 (English)In: Handbook of Metal-Microbe Interactions and Bioremediation / [ed] Surajit Das, Hirak Ranjan Dash, Boca Raton, FL: CRC Press, 2017, p. 509-519Chapter in book (Refereed)
Abstract [en]

In this chapter, arsenic-accumulating and arsenic- transformingbacterial strains that can be employed as a sourcefor cost-effective and eco-friendly bioremediation of arsenicsfrom contaminated environments have been reviewed. Thischapter demonstrates that many naturally occurring bacterialstrains like B1-CDA have the potential for reducing arseniccontent in contaminated sources to safe levels. Therefore,the socioeconomic impact of this kind of microorganisms ishighly significant for those countries, especially in the developingworld, where impoverished families and villages aremost impacted. Therefore, this discovery should be consideredto be the most significant factor in formulating nationalstrategies for effective poverty elimination. Besides humanarsenic contamination, these bacterial strains will also benefitlivestock and native animal species, and the outcome ofthese studies is vital not only for people in arsenic-affectedareas but also for human populations in other countries thathave credible health concerns as a consequence of arseniccontaminatedwater and foods.

Place, publisher, year, edition, pages
Boca Raton, FL: CRC Press, 2017
National Category
Microbiology
Research subject
Biotechnology
Identifiers
urn:nbn:se:his:diva-14275 (URN)2-s2.0-85053344387 (Scopus ID)9781498762427 (ISBN)9781498762434 (ISBN)
Available from: 2017-10-30 Created: 2017-10-30 Last updated: 2019-09-05Bibliographically approved
Nahar, N., Rahman, A., Nawani, N. N., Ghosh, S. & Mandal, A. (2017). Phytoremediation of arsenic from the contaminated soil using transgenic tobacco plants expressing ACR2 gene of Arabidopsis thaliana. Journal of plant physiology (Print), 218, 121-126
Open this publication in new window or tab >>Phytoremediation of arsenic from the contaminated soil using transgenic tobacco plants expressing ACR2 gene of Arabidopsis thaliana
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2017 (English)In: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 218, p. 121-126Article in journal (Refereed) Published
Abstract [en]

We have cloned, characterized and transformed the AtACR2 gene (arsenic reductase 2) of Arabidopsis thaliana into the genome of tobacco (Nicotiana tabacum, var Sumsun). Our results revealed that the transgenic tobacco plants are more tolerant to arsenic than the wild type ones. These plants can grow on culture medium containing 200μM arsenate, whereas the wild type can barely survive under this condition. Furthermore, when exposed to 100μM arsenate for 35days the amount of arsenic accumulated in the shoots of transgenic plants was significantly lower (28μg/g d wt.) than that found in the shoots of non-transgenic controls (40μg/g d wt.). However, the arsenic content in the roots of transgenic plants was significantly higher (2400μg/g d. wt.) than that (2100μg/g d. wt.) observed in roots of wild type plants. We have demonstrated that Arabidopsis thaliana AtACR2 gene is a potential candidate for genetic engineering of plants to develop new crop cultivars that can be grown on arsenic contaminated fields to reduce arsenic content of the soil and can become a source of food containing no arsenic or exhibiting substantially reduced amount of this metalloid.

Place, publisher, year, edition, pages
Elsevier, 2017
Keywords
Arabidopsis thaliana, Arsenic, AtACR2 overexpression, Heavy metal accumulation, Nicotiana tabacum, Phytoremediation
National Category
Botany Biochemistry and Molecular Biology
Research subject
Biotechnology
Identifiers
urn:nbn:se:his:diva-14271 (URN)10.1016/j.jplph.2017.08.001 (DOI)000413327800014 ()28818758 (PubMedID)2-s2.0-85031780264 (Scopus ID)
Available from: 2017-10-30 Created: 2017-10-30 Last updated: 2018-02-16Bibliographically approved
Yewale, P. P., Rahman, A., Nahar, N., Saha, A., Jass, J., Mandal, A. & Nawani, N. N. (2017). Sources of Metal Pollution, Global Status, and Conventional Bioremediation Practices. In: Surajit Das, Hirak Ranjan Dash (Ed.), Handbook of Metal–Microbe Interactions and Bioremediation: (pp. 25-40). Boca Raton, FL: CRC Press
Open this publication in new window or tab >>Sources of Metal Pollution, Global Status, and Conventional Bioremediation Practices
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2017 (English)In: Handbook of Metal–Microbe Interactions and Bioremediation / [ed] Surajit Das, Hirak Ranjan Dash, Boca Raton, FL: CRC Press, 2017, p. 25-40Chapter in book (Refereed)
Abstract [en]

Pollution control has become a priority task for global regulatory authorities. The framing of regulations, guidelines, and implementation of pollution awareness and control programs has begun at a massive scale. Heavy metals that are one of the most challenging pollutants that affect humans, animals, plants, and the ecosystem health. The sources of different metals and their toxicities are described. Current approaches in bioremediation are addressed along with the challenges posed by them. Furthermore, recent developments in biotechnology that offer novel ways to recover metals from contaminated sites are discussed.

Place, publisher, year, edition, pages
Boca Raton, FL: CRC Press, 2017
Keywords
Metal Pollution, Bioremediation, Human Health, Microbial Biotechnology
National Category
Natural Sciences Biological Sciences Microbiology
Research subject
Biotechnology
Identifiers
urn:nbn:se:his:diva-14283 (URN)2-s2.0-85043683459 (Scopus ID)9781498762427 (ISBN)9781498762434 (ISBN)
Available from: 2017-11-01 Created: 2017-11-01 Last updated: 2019-09-05Bibliographically approved
Rahman, A., Nahar, N., Olsson, B. & Mandal, A. (2016). Complete Genome Sequence of Enterobacter cloacae B2-DHA: a Chromium-Resistant Bacterium. Genome Announcements, 4(3), Article ID e00483-16.
Open this publication in new window or tab >>Complete Genome Sequence of Enterobacter cloacae B2-DHA: a Chromium-Resistant Bacterium
2016 (English)In: Genome Announcements, ISSN 2169-8287, E-ISSN 2169-8287, Vol. 4, no 3, article id e00483-16Article in journal (Refereed) Published
Abstract [en]

Previously, we reported a chromium-resistant bacterium, Enterobacter cloacae B2-DHA, isolated from the landfills of tannery industries in Bangladesh. Here, we investigated its genetic composition using massively parallel sequencing and comparative analysis with other known Enterobacter genomes. Assembly of the sequencing reads revealed a genome of ~4.21 Mb in size.

Place, publisher, year, edition, pages
American Society for Microbiology, 2016
Keywords
Enterobacter cloacae, Genome sequencing, de novo assembly, Gene annotation
National Category
Bioinformatics and Systems Biology
Research subject
Bioinformatics; Biotechnology
Identifiers
urn:nbn:se:his:diva-12317 (URN)10.1128/genomeA.00483-16 (DOI)000460660100145 ()27257201 (PubMedID)2-s2.0-85009965114 (Scopus ID)
Available from: 2016-06-01 Created: 2016-06-01 Last updated: 2019-03-25Bibliographically approved
Rahman, A., Nahar, N., Jass, J., Olsson, B. & Mandal, A. (2016). Complete genome sequence of Lysinibacillus sphaericus B1-CDA: a bacterium that accumulates arsenics. Genome Announcements, 4(1), Article ID e00999-15.
Open this publication in new window or tab >>Complete genome sequence of Lysinibacillus sphaericus B1-CDA: a bacterium that accumulates arsenics
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2016 (English)In: Genome Announcements, ISSN 2169-8287, E-ISSN 2169-8287, Vol. 4, no 1, article id e00999-15Article in journal (Refereed) Published
Abstract [en]

Here, we report the genomic sequence and genetic composition of an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA. Assembly of the sequencing reads revealed that the genome size is ~4.5 Mb encompassing ~80% of the chromosomal DNA.

Place, publisher, year, edition, pages
American Society for Microbiology, 2016
National Category
Bioinformatics and Systems Biology
Research subject
Natural sciences; Bioinformatics
Identifiers
urn:nbn:se:his:diva-11733 (URN)10.1128/genomeA.00999-15 (DOI)000460649500018 ()26798084 (PubMedID)2-s2.0-85009977094 (Scopus ID)
Available from: 2015-12-01 Created: 2015-12-01 Last updated: 2019-03-25Bibliographically approved
Nawani, N., Rahman, A., Nahar, N., Saha, A., Kapadnis, B. & Mandal, A. (2016). Status of metal pollution in rivers flowing through urban settlements at Pune and its effect on resident microflora. Biologia (Bratislava), 71(5), 494-507
Open this publication in new window or tab >>Status of metal pollution in rivers flowing through urban settlements at Pune and its effect on resident microflora
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2016 (English)In: Biologia (Bratislava), ISSN 0006-3088, E-ISSN 1336-9563, Vol. 71, no 5, p. 494-507Article in journal (Refereed) Published
Abstract [en]

This study illustrates the sporadic distribution of metals in fluvial systems flowing from catchments to urban settlements. This is a detailed study prognosticating the deteriorating quality of rivers at specific locations due to metal pollution. Heavy metals like cadmium, lead, nickel and mercury are prominent in industrial sector. Contour plots derived using spatial and temporal data could determine the focal point of metal pollution and its gradation. Metal values recorded were cadmium 157 mg/L, lead 47 mg/L, nickel 61 mg/L and mercury 0.56 mg/L. Prokaryote diversity was less in polluted water and it harboured metal tolerant bacteria, which were isolated from these polluted sites. Actinomycetes like Streptomyces and several other bacteria like Stenotrophomonas and Pseudomonas isolated from the polluted river sites exhibited changes in morphology in presence of heavy metals. This stress response offered remedial measures as Streptomyces were effective in biosorption of cadmium, nickel and lead and Stenotrophomonas and Pseudomonas were effective in the bioaccumulation of lead and cadmium. The amount of 89 mg of lead and 106 mg of nickel could be adsorbed on one gram of Streptomyces biomass-based biosorbent. Such biological remedies can be further explored to remove metals from polluted sites and from metal contaminated industrial or waste waters.

Place, publisher, year, edition, pages
Bratislava: Springer, 2016
Keywords
Metal pollution, Bioremediation, Morphological Changes, Pune rivers, Prokaryote Diversity
National Category
Microbiology Environmental Sciences Water Engineering
Research subject
Natural sciences; Biotechnology
Identifiers
urn:nbn:se:his:diva-12307 (URN)10.1515/biolog-2016-0074 (DOI)000379818000005 ()2-s2.0-84976553017 (Scopus ID)
Projects
Bioremediation of toxic metals and other pollutants for protecting human health and the ecosystem
Funder
Sida - Swedish International Development Cooperation Agency
Available from: 2016-05-31 Created: 2016-05-31 Last updated: 2019-11-25Bibliographically approved
Rahman, A., Nahar, N., Nawani, N. N., Jass, J., Hossain, K., Alam Saud, Z., . . . Mandal, A. (2015). Bioremediation of hexavalent chromium (VI) by a soil borne bacterium, Enterobacter cloacae B2-DHA. Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering, 50(11), 1136-1147
Open this publication in new window or tab >>Bioremediation of hexavalent chromium (VI) by a soil borne bacterium, Enterobacter cloacae B2-DHA
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2015 (English)In: Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering, ISSN 1093-4529, E-ISSN 1532-4117, Vol. 50, no 11, p. 1136-1147Article in journal (Refereed) Published
Abstract [en]

Chromium and chromium containing compounds are discharged into the nature as waste from anthropogenic activities, such as industries, agriculture, forest farming, mining and metallurgy. Continued disposal of these compounds to the environment leads to development of various lethal diseases in both humans and animals. In this paper, we report a soil borne bacterium, B2-DHA that can be used as a vehicle to effectively remove chromium from the contaminated sources. B2-DHA is resistant to chromium with a MIC value of 1000 µg/mL potassium chromate. The bacterium has been identified as a Gram negative, Enterobacter cloacae based on biochemical characteristics and 16S rRNA gene analysis. TOF-SIMS and ICP-MS analyses confirmed intracellular accumulation of chromium and thus its removal from the contaminated liquid medium. Chromium accumulation in cells was 320 µg/g of cells dry biomass after 120 h exposure and thus it reduced the chromium concentration in the liquid medium by as much as 81%. Environmental scanning electron micrograph revealed the effect of metals on cellular morphology of the isolates. Altogether, our results indicate that B2-DHA has the potential to reduce chromium significantly to safe levels from the contaminated environments and suggest the potential use of this bacterium in reducing human exposure to chromium, hence avoiding poisoning.

Place, publisher, year, edition, pages
Taylor & Francis, 2015
Keywords
Bioremediation, Chromium, Enterobacter cloacae, Human Health, Tannery Effluents, Soil Borne Bacterium
National Category
Microbiology
Research subject
Bioinformatics
Identifiers
urn:nbn:se:his:diva-10916 (URN)10.1080/10934529.2015.1047670 (DOI)000359339900006 ()26191988 (PubMedID)2-s2.0-84937800926 (Scopus ID)
Available from: 2015-05-11 Created: 2015-05-11 Last updated: 2018-07-31Bibliographically approved
Rahman, A., Nahar, N., Nawani, N. N., Jass, J., Ghosh, S., Olsson, B. & Mandal, A. (2015). Comparative genome analysis of Lysinibacillus B1-CDA, a bacterium that accumulates arsenics. Genomics, 106(6), 384-392
Open this publication in new window or tab >>Comparative genome analysis of Lysinibacillus B1-CDA, a bacterium that accumulates arsenics
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2015 (English)In: Genomics, ISSN 0888-7543, E-ISSN 1089-8646, Vol. 106, no 6, p. 384-392Article in journal (Refereed) Published
Abstract [en]

Previously, we reported an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, isolated from an arsenic contaminated lands. Here, we have investigated its genetic composition and evolutionary history by using massively parallel sequencing and comparative analysis with other known Lysinibacillus genomes. Assembly of the sequencing reads revealed a genome of ~ 4.5 Mb in size encompassing ~ 80% of the chromosomal DNA. We found that the set of ordered contigs contains abundant regions of similarity with other Lysinibacillus genomes and clearly identifiable genome rearrangements. Furthermore, all genes of B1-CDA that were predicted be involved in its resistance to arsenic and/or other heavy metals were annotated. The presence of arsenic responsive genes was verified by PCR in vitro conditions. The findings of this study highlight the significance of this bacterium in removing arsenics and other toxic metals from the contaminated sources. The genetic mechanisms of the isolate could be used to cope with arsenic toxicity.

Place, publisher, year, edition, pages
Elsevier, 2015
Keywords
Toxic metals, Bioremediation, Genome sequencing, de novo assembly
National Category
Biochemistry and Molecular Biology Bioinformatics and Systems Biology
Research subject
Natural sciences; Bioinformatics; Biotechnology
Identifiers
urn:nbn:se:his:diva-11575 (URN)10.1016/j.ygeno.2015.09.006 (DOI)000365613100010 ()26387925 (PubMedID)2-s2.0-84948102629 (Scopus ID)
Projects
Bioremediation
Funder
Sida - Swedish International Development Cooperation Agency, AKT-2010-18
Available from: 2015-10-02 Created: 2015-10-01 Last updated: 2018-07-31Bibliographically approved
Rahman, A., Nahar, N., Nawani, N. N., Jass, J., Ghosh, S., Olsson, B. & Mandal, A. (2015). Data in support of the comparative genome analysis of Lysinibacillus B1-CDA, a bacterium that accumulates arsenics. Data in Brief, 5, 579-585
Open this publication in new window or tab >>Data in support of the comparative genome analysis of Lysinibacillus B1-CDA, a bacterium that accumulates arsenics
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2015 (English)In: Data in Brief, ISSN 2352-3409, Vol. 5, p. 579-585Article in journal (Refereed) Published
Abstract [en]

This study is a part of our long term project on bioremediation of toxic metals and other pollutants for protection of human health and the environment from severe contamination. The information and results presented in this data article are based on both in vitro and in silico experiments. In vitro experiments were used to investigate the presence of arsenic responsive genes in a bacterial strain B1-CDA that is highly resistant to arsenics. However, in silico studies were used to annotate the function of the metal responsive genes. By using this combined study consisting of in vitro and in silico experiments we have identified and characterized specific genes from B1-CDA that can be used as a potential tool for removal of arsenics as well as other heavy metals from the contaminated environment.

Place, publisher, year, edition, pages
Elsevier, 2015
Keywords
Bacteria, Arsenic, Bioremediation, Genome, De novo assembly, gene prediction
National Category
Bioinformatics and Systems Biology Biochemistry and Molecular Biology
Research subject
Natural sciences; Biotechnology; Bioinformatics
Identifiers
urn:nbn:se:his:diva-11576 (URN)10.1016/j.dib.2015.09.040 (DOI)000453160000095 ()26387925 (PubMedID)2-s2.0-84945246394 (Scopus ID)
Available from: 2015-10-02 Created: 2015-10-01 Last updated: 2019-03-25Bibliographically approved
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