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Potter, Ryan
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Potter, R., Ayala, M. & Tilevik, A. (2024). Identification of biomarker candidates for exfoliative glaucoma from autoimmunity profiling. BMC Ophthalmology, 24(1), Article ID 44.
Open this publication in new window or tab >>Identification of biomarker candidates for exfoliative glaucoma from autoimmunity profiling
2024 (English)In: BMC Ophthalmology, E-ISSN 1471-2415, Vol. 24, no 1, article id 44Article in journal (Refereed) Published
Abstract [en]

Background: Exfoliative glaucoma (XFG) is a subtype of open-angle glaucoma characterized by distinctive extracellular fibrils and a yet unknown pathogenesis potentially involving immune-related factors. The aim of this exploratory study was to identify biomarkers for XFG using data from autoimmunity profiling performed on blood samples from a Scandinavian cohort of patients. Methods: Autoantibody screening was analyzed against 258 different protein fragments in blood samples taken from 30 patients diagnosed with XFG and 30 healthy donors. The 258 protein fragments were selected based on a preliminary study performed on 3072 randomly selected antigens and antigens associated with the eye. The “limma” package was used to perform moderated t-tests on the proteomic data to identify differentially expressed reactivity between the groups. Results: Multiple associated genes were highlighted as possible biomarker candidates including FUT2, CDH5, and the LOX family genes. Using seven variables, our binary logistic regression model was able to classify the cases from the controls with an AUC of 0.85, and our reduced model using only one variable corresponding to the FUT2 gene provided an AUC of 0.75, based on LOOCV. Furthermore, over-representation gene analysis was performed to identify pathways that were associated with antigens differentially bound to self-antibodies. This highlighted the enrichment of pathways related to collagen fibril formation and the regulatory molecules mir-3176 and mir-876-5p. Conclusions: This study suggests several potential biomarkers that may be useful in developing further models of the pathology of XFG. In particular, CDH5, FUT2, and the LOX family seem to have a relationship which merits additional exploration. 

Place, publisher, year, edition, pages
BioMed Central (BMC), 2024
Keywords
Autoimmunity, Biomarkers, Exfoliation, Genes, Glaucoma
National Category
Ophthalmology Medical Genetics Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Bioinformatics and Systems Biology
Research subject
Infection Biology
Identifiers
urn:nbn:se:his:diva-23583 (URN)10.1186/s12886-024-03314-y (DOI)001153831500002 ()38287276 (PubMedID)2-s2.0-85183441039 (Scopus ID)
Funder
University of Gothenburg
Note

CC BY 4.0 DEED

© 2024, The Author(s).

Correspondence Address: R. Potter; Sahlgrenska Academy, Gothenburg University, Gothenburg, Sweden; email: ryan.potter@his.se

Open access funding provided by University of Gothenburg. This study was funded by Synskadades Vänner Skaraborg (SVS).

Available from: 2024-02-08 Created: 2024-02-08 Last updated: 2024-04-15Bibliographically approved
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